Characterization of inulin-type fructans from two species of Radix Codonopsis and their oxidative defense activation and prebiotic activities.
Codonopsis pilosula
Codonopsis tangshen
antioxidation
inulin-type fructans
prebiotic activity
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
revised:
15
09
2020
received:
24
03
2020
accepted:
15
10
2020
pubmed:
17
10
2020
medline:
28
4
2021
entrez:
16
10
2020
Statut:
ppublish
Résumé
Codonopsis pilosula and C. tangshen are both plants widely used in traditional Chinese medicine. Polysaccharides, which are their primary active components, are thought to be important in their extensive use. In this study, two neutral polysaccharide fractions of C. pilosula (CPPN) and C. tangshen (CTPN) were obtained by fractionation on a DEAE-Sepharose column and characterized. It was confirmed that the neutral polymers CPPN and CTPN were β-(2,1)-linked inulin-type fructans with non-reducing terminal glucose, and degree of polymerization (DP) of 19.6 and 25.2, respectively. The antioxidant and prebiotic activities in vitro were assayed based on IPEC-J2 cell lines and five strains of Lactobacillus. Results indicated that the effects of CPPN and CTPN were increased antioxidant defense in intestinal epithelial cells through enhanced cell viability, improved expression of total antioxidant capacity, glutathione peroxidase, superoxide dismutase and catalase, and reduced levels of malondialdehyde and lactic dehydrogenase. The prebiotic activity of CPPN and CTPN was demonstrated by the promoting effect on Lactobacillus proliferation in vitro. The different biological activities obtained between the two fractions are probably due to the different DP and thus molecular weights of CPPN and CTPN. The inulin fractions from C. pilosula and C. tangshen were natural sources of potential intestinal antioxidants as well as prebiotics, which will be valuable in further studies and new applications of inulin-containing health products. © 2020 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Codonopsis pilosula and C. tangshen are both plants widely used in traditional Chinese medicine. Polysaccharides, which are their primary active components, are thought to be important in their extensive use. In this study, two neutral polysaccharide fractions of C. pilosula (CPPN) and C. tangshen (CTPN) were obtained by fractionation on a DEAE-Sepharose column and characterized.
RESULTS
RESULTS
It was confirmed that the neutral polymers CPPN and CTPN were β-(2,1)-linked inulin-type fructans with non-reducing terminal glucose, and degree of polymerization (DP) of 19.6 and 25.2, respectively. The antioxidant and prebiotic activities in vitro were assayed based on IPEC-J2 cell lines and five strains of Lactobacillus. Results indicated that the effects of CPPN and CTPN were increased antioxidant defense in intestinal epithelial cells through enhanced cell viability, improved expression of total antioxidant capacity, glutathione peroxidase, superoxide dismutase and catalase, and reduced levels of malondialdehyde and lactic dehydrogenase. The prebiotic activity of CPPN and CTPN was demonstrated by the promoting effect on Lactobacillus proliferation in vitro. The different biological activities obtained between the two fractions are probably due to the different DP and thus molecular weights of CPPN and CTPN.
CONCLUSION
CONCLUSIONS
The inulin fractions from C. pilosula and C. tangshen were natural sources of potential intestinal antioxidants as well as prebiotics, which will be valuable in further studies and new applications of inulin-containing health products. © 2020 Society of Chemical Industry.
Substances chimiques
Antioxidants
0
Drugs, Chinese Herbal
0
Fructans
0
Prebiotics
0
Inulin
9005-80-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2491-2499Subventions
Organisme : General Financial Grant from the China Postdoctoral Science Foundation
ID : 2016M602704
Organisme : International Cooperation Projects of Science & Technology Department of Sichuan Province
ID : 2017HH0093
Organisme : Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System
ID : SCCXTD-2020-18
Informations de copyright
© 2020 Society of Chemical Industry.
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