Hepatoma-derived growth factor participates in concanavalin A-induced hepatitis.
Alanine Transaminase
/ metabolism
Animals
Aspartate Aminotransferases
/ metabolism
Cells, Cultured
Concanavalin A
/ pharmacology
Hepatitis, Animal
/ chemically induced
Hepatocytes
/ drug effects
Intercellular Signaling Peptides and Proteins
/ metabolism
Interleukin-1beta
/ metabolism
Interleukin-6
/ metabolism
Liver
/ drug effects
Liver Cirrhosis
/ metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Neutrophil Infiltration
/ drug effects
Rats
Signal Transduction
/ drug effects
Tumor Necrosis Factor-alpha
/ metabolism
Up-Regulation
/ drug effects
acute hepatitis
concanavalin A
hepatoma-derived growth factor
neutrophils
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
06
03
2020
revised:
13
09
2020
accepted:
28
09
2020
pubmed:
17
10
2020
medline:
24
4
2021
entrez:
16
10
2020
Statut:
ppublish
Résumé
Hepatitis is an important health problem worldwide. Novel molecular targets are in demand for detection and management of hepatitis. Hepatoma-derived growth factor (HDGF) has been delineated to participate in hepatic fibrosis and liver carcinogenesis. However, the relationship between hepatitis and HDGF remains unclear. This study aimed to elucidate the role of HDGF during hepatitis using concanavalin A (ConA)-induced hepatitis model. In cultured hepatocytes, ConA treatment-elicited HDGF upregulation at transcriptional level and promoted HDGF secretion while reducing intracellular HDGF protein level and cellular viability. Similarly, mice receiving ConA administration exhibited reduced hepatic HDGF expression and elevated circulating HDGF level, which was positively correlated with serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. By using HDGF knockout (KO) mice, it was found the ConA-evoked cell death was prominently alleviated in KO compared with control. Besides, it was delineated HDGF ablation conferred protection by suppressing the ConA-induced neutrophils recruitment in livers. Above all, the ConA-mediated activation of tumor necrosis factor-α (TNF-α)/interleukin-1β (IL-1β)/interleukin-6 (IL-6)/cyclooxygenase-2 (COX-2) inflammatory signaling was significantly abrogated in KO mice. Treatment with recombinant HDGF (rHDGF) dose-dependently stimulated the expression of TNF-α/IL-1β/IL-6/COX-2 in hepatocytes, further supporting the pro-inflammatory function of HDGF. Finally, application of HDGF antibody not only attenuated the ConA-mediated inflammatory cascade in hepatocytes, but also ameliorated the ConA-induced hepatic necrosis and AST elevation in mice. In summary, HDGF participates in ConA-induced hepatitis via neutrophils recruitment and may constitute a therapeutic target for acute hepatitis.
Identifiants
pubmed: 33063394
doi: 10.1096/fj.202000511RR
doi:
Substances chimiques
Intercellular Signaling Peptides and Proteins
0
Interleukin-1beta
0
Interleukin-6
0
Tumor Necrosis Factor-alpha
0
hepatoma-derived growth factor
0
Concanavalin A
11028-71-0
Aspartate Aminotransferases
EC 2.6.1.1
Alanine Transaminase
EC 2.6.1.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16163-16178Informations de copyright
© 2020 Federation of American Societies for Experimental Biology.
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