Prevention of LPS-induced acute respiratory distress syndrome in sheep by bone marrow-derived mesenchymal stem/stromal cells.
ARDS
BM-MSCs
Lung
Sheep
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
15 Dec 2020
15 Dec 2020
Historique:
received:
09
09
2020
revised:
04
10
2020
accepted:
11
10
2020
pubmed:
18
10
2020
medline:
16
12
2020
entrez:
17
10
2020
Statut:
ppublish
Résumé
In this study, 10 male Shall sheep were used in two groups and bone marrow samples were collected and BM-MSCs isolated. Then experimental model of ARDS was induced by intrapulmonary injection of LPS to dose of 400 μg/kg. Twenty-four hours after LPS injection, 5 × 10 The results of the investigations 24 h post-LPS injection(time 0) indicated the occurrence of acute inflammation which confirmed ARDS model. These changes included increase in RR, HR and RT, decrease in PO2 and SatO2 and increase in PCO2, WBC, neutrophils, macrophages, total protein,IL6,IL10, TNF-α,Ig M and albumin. But the stem/stromal cells transplantation reduced the severity of clinical signs induced by LPS, caused significant increase in PO These results showed that BM-MSCs are able to reduce inflammation and have an important role in reconstruction of the damaged lung.
Identifiants
pubmed: 33068598
pii: S0024-3205(20)31353-9
doi: 10.1016/j.lfs.2020.118600
pii:
doi:
Substances chimiques
Cytokines
0
Lipopolysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
118600Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.