Performance of a multiplex polymerase chain reaction panel for identifying bacterial pathogens causing pneumonia in critically ill patients with COVID-19.
Aged
Bacteria
/ classification
Bacteriological Techniques
/ methods
Bronchoalveolar Lavage Fluid
/ microbiology
COVID-19
/ complications
Critical Illness
Female
Humans
Male
Middle Aged
Multiplex Polymerase Chain Reaction
/ methods
Pneumonia, Bacterial
/ diagnosis
SARS-CoV-2
Sensitivity and Specificity
Time Factors
Bacterial pneumonia
FilmArray Pneumonia Plus
Rapid diagnostics
SARS-CoV-2
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
24
05
2020
revised:
10
08
2020
accepted:
13
08
2020
pubmed:
18
10
2020
medline:
19
12
2020
entrez:
17
10
2020
Statut:
ppublish
Résumé
The FilmArray® Pneumonia Plus (FA-PP) panel can provide rapid identifications and semiquantitative results for many pathogens. We performed a prospective single-center study in 43 critically ill patients with coronavirus disease 2019 (COVID-19) in which we performed 96 FA-PP tests and cultures of blind bronchoalveolar lavage (BBAL). FA-PP detected 1 or more pathogens in 32% (31/96 of samples), whereas culture methods detected at least 1 pathogen in 35% (34/96 of samples). The most prevalent bacteria detected were Pseudomonas aeruginosa (n = 14) and Staphylococcus aureus (n = 11) on both FA-PP and culture. The FA-PP results from BBAL in critically ill patients with COVID-19 were consistent with bacterial culture findings for bacteria present in the FA-PP panel, showing sensitivity, specificity, and positive and negative predictive value of 95%, 99%, 82%, and 100%, respectively. Median turnaround time for FA-PP was 5.5 h, which was significantly shorter than for standard culture (26 h) and antimicrobial susceptibility testing results (57 h).
Identifiants
pubmed: 33069002
pii: S0732-8893(20)30560-5
doi: 10.1016/j.diagmicrobio.2020.115183
pmc: PMC7441025
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115183Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Competing interests F.C. received conference invitations from BioMérieux.
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