Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand.
Anopheles hyrcanus
Insecticide resistance
Malaria vectors
Species diversity
Thailand
Ubon Ratchathani Province
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
17 Oct 2020
17 Oct 2020
Historique:
received:
19
06
2020
accepted:
06
10
2020
entrez:
18
10
2020
pubmed:
19
10
2020
medline:
1
6
2021
Statut:
epublish
Résumé
Members of the Anopheles hyrcanus group have been incriminated as important malaria vectors. This study aims to identify the species and explore the insecticide susceptibility profile within the Anopheles hyrcanus group in Ubon Ratchathani Province, northeastern Thailand where increasing numbers of malaria cases were reported in 2014. Between 2013 and 2015, five rounds of mosquito collections were conducted using human landing and cattle bait techniques during both the rainy and dry seasons. Anopheles mosquitoes were morphologically identified and their insecticide susceptibility status was investigated. Synergist bioassays were carried out with An. hyrcanus (s.l.) due to their resistance to all insecticides. An ITS2-PCR assay was conducted to identify to species the Hyrcanus group specimens. Out of 10,361 Anopheles females collected, representing 18 taxa in 2 subgenera, 71.8% were morphologically identified as belonging to the Hyrcanus Group (subgenus Anopheles), followed by An. barbirostris group (7.9%), An. nivipes (6.5%), An. philippinensis (5.9%) and the other 14 Anopheles species. Specimens of the Hyrcanus Group were more prevalent during the rainy season and were found to be highly zoophilic. Anopheles hyrcanus (s.l.) was active throughout the night, with an early peak of activity between 18:00 h and 21:00 h. ITS2-PCR assay conducted on 603 DNA samples from specimens within the Hyrcanus Group showed the presence of five sisters species. Anopheles peditaeniatus was the most abundant species (90.5%, n = 546), followed by An. nitidus (4.5%, n = 27), An. nigerrimus (4.3%, n = 26), An. argyropus (0.5%, n = 3), and An. sinensis (0.2%, n = 1). All An. hyrcanus (s.l.) specimens that were found resistant to insecticides (deltamethrin 0.05%, permethrin 0.75% and DDT 4% and synergist tests) belonged to An. peditaeniatus. The degree of resistance in An. peditaeniatus to each of these three insecticides was approximately 50%. Addition of PBO (Piperonyl butoxide), but not DEF (S.S.S-tributyl phosphotritioate), seemed to restore susceptibility, indicating a potential role of oxidases as a detoxifying enzyme resistance mechanism. A better understanding of mosquito diversity related to host preference, biting activity and insecticide resistance status will facilitate the implementation of locally adapted vector control strategies.
Sections du résumé
BACKGROUND
BACKGROUND
Members of the Anopheles hyrcanus group have been incriminated as important malaria vectors. This study aims to identify the species and explore the insecticide susceptibility profile within the Anopheles hyrcanus group in Ubon Ratchathani Province, northeastern Thailand where increasing numbers of malaria cases were reported in 2014.
METHODS
METHODS
Between 2013 and 2015, five rounds of mosquito collections were conducted using human landing and cattle bait techniques during both the rainy and dry seasons. Anopheles mosquitoes were morphologically identified and their insecticide susceptibility status was investigated. Synergist bioassays were carried out with An. hyrcanus (s.l.) due to their resistance to all insecticides. An ITS2-PCR assay was conducted to identify to species the Hyrcanus group specimens.
RESULTS
RESULTS
Out of 10,361 Anopheles females collected, representing 18 taxa in 2 subgenera, 71.8% were morphologically identified as belonging to the Hyrcanus Group (subgenus Anopheles), followed by An. barbirostris group (7.9%), An. nivipes (6.5%), An. philippinensis (5.9%) and the other 14 Anopheles species. Specimens of the Hyrcanus Group were more prevalent during the rainy season and were found to be highly zoophilic. Anopheles hyrcanus (s.l.) was active throughout the night, with an early peak of activity between 18:00 h and 21:00 h. ITS2-PCR assay conducted on 603 DNA samples from specimens within the Hyrcanus Group showed the presence of five sisters species. Anopheles peditaeniatus was the most abundant species (90.5%, n = 546), followed by An. nitidus (4.5%, n = 27), An. nigerrimus (4.3%, n = 26), An. argyropus (0.5%, n = 3), and An. sinensis (0.2%, n = 1). All An. hyrcanus (s.l.) specimens that were found resistant to insecticides (deltamethrin 0.05%, permethrin 0.75% and DDT 4% and synergist tests) belonged to An. peditaeniatus. The degree of resistance in An. peditaeniatus to each of these three insecticides was approximately 50%. Addition of PBO (Piperonyl butoxide), but not DEF (S.S.S-tributyl phosphotritioate), seemed to restore susceptibility, indicating a potential role of oxidases as a detoxifying enzyme resistance mechanism.
CONCLUSIONS
CONCLUSIONS
A better understanding of mosquito diversity related to host preference, biting activity and insecticide resistance status will facilitate the implementation of locally adapted vector control strategies.
Identifiants
pubmed: 33069255
doi: 10.1186/s13071-020-04389-4
pii: 10.1186/s13071-020-04389-4
pmc: PMC7568835
doi:
Substances chimiques
Insecticides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
525Références
J Med Entomol. 2005 Jul;42(4):610-9
pubmed: 16119550
J Med Entomol. 2004 Jan;41(1):5-11
pubmed: 14989340
Trop Med Int Health. 2005 Mar;10(3):251-62
pubmed: 15730510
J Med Entomol. 2016 Nov;53(6):1422-1424
pubmed: 27838614
Acta Trop. 2013 Dec;128(3):623-9
pubmed: 24055545
Korean J Parasitol. 2010 Mar;48(1):35-41
pubmed: 20333283
Parasit Vectors. 2010 Jul 21;3(1):59
pubmed: 20646327
J Am Mosq Control Assoc. 2000 Sep;16(3):189-98
pubmed: 11081645
J Vector Ecol. 2014 Dec;39(2):361-71
pubmed: 25424266
Chin Med J (Engl). 1984 Aug;97(8):609-12
pubmed: 6440752
Parasit Vectors. 2017 Mar 31;10(1):165
pubmed: 28359289
Parasit Vectors. 2013 Feb 22;6:41
pubmed: 23433176
Malar J. 2016 Apr 26;15:242
pubmed: 27118141
Trans R Soc Trop Med Hyg. 1994 Sep-Oct;88(5):510-2
pubmed: 7992323
Parasit Vectors. 2013 Jun 14;6:176
pubmed: 23768077
J Med Entomol. 1990 Nov;27(6):1016-26
pubmed: 2280385
Malar J. 2010 Jul 07;9:193
pubmed: 20609220
Malar J. 2007 Mar 06;6:26
pubmed: 17341297
Am J Trop Med Hyg. 1996 Feb;54(2):114-21
pubmed: 8619432
Mol Ecol Resour. 2010 Jul;10(4):748-50
pubmed: 21565085
Malar J. 2010 Jan 14;9:15
pubmed: 20074326
Parasit Vectors. 2013 Nov 19;6(1):332
pubmed: 24252367
Indian J Med Res. 2003 Mar;117:104-10
pubmed: 14575175
Southeast Asian J Trop Med Public Health. 1994 Dec;25(4):766-70
pubmed: 7667729
Southeast Asian J Trop Med Public Health. 1999 Mar;30(1):184-94
pubmed: 10695809
Am J Trop Med Hyg. 2007 Mar;76(3):450-60
pubmed: 17360867
Int J Parasitol. 2002 Dec 4;32(13):1607-15
pubmed: 12435445
Mol Phylogenet Evol. 2004 Jan;30(1):236-42
pubmed: 15022773
Malar J. 2011 Mar 14;10:59
pubmed: 21401946
J Hered. 2007 Jan-Feb;98(1):51-9
pubmed: 17158469
Malar J. 2016 Aug 30;15(1):440
pubmed: 27577697
Korean J Parasitol. 2002 Jun;40(2):75-81
pubmed: 12073732
Malar J. 2017 Jul 25;16(1):299
pubmed: 28743278
Trends Parasitol. 2017 Nov;33(11):889-900
pubmed: 28734898
Med Vet Entomol. 2002 Mar;16(1):46-54
pubmed: 11963981
Malar J. 2008 Jun 05;7:102
pubmed: 18534006
Mol Phylogenet Evol. 2009 Jan;50(1):141-51
pubmed: 19000771
Zhonghua Yu Fang Yi Xue Za Zhi. 1990 Sep;24(5):265-7
pubmed: 1979768
Infect Genet Evol. 2010 Mar;10(2):159-77
pubmed: 19941975
Parasit Vectors. 2011 May 25;4:89
pubmed: 21612587
Wellcome Open Res. 2018 Aug 31;3:109
pubmed: 31206035
Parasitol Res. 2017 Dec;116(12):3349-3359
pubmed: 29082435
Parasit Vectors. 2013 Jan 04;6:5
pubmed: 23289957
Front Microbiol. 2017 Aug 09;8:1473
pubmed: 28848504
J Am Mosq Control Assoc. 2001 Mar;17(1):67-72
pubmed: 11345422
Trans R Soc Trop Med Hyg. 1989 Jul-Aug;83(4):550-2
pubmed: 2575809
Malar J. 2010 Sep 16;9:259
pubmed: 20846447
Southeast Asian J Trop Med Public Health. 1998 Mar;29(1):159-67
pubmed: 9740293
Southeast Asian J Trop Med Public Health. 2006;37 Suppl 2:1-128
pubmed: 17262930
Korean J Parasitol. 1999 Sep;37(3):129-43
pubmed: 10507220
Parasit Vectors. 2014 Jun 09;7:265
pubmed: 24912923
J Insect Sci. 2011;11:10
pubmed: 21521137
J Vector Ecol. 2017 Dec;42(2):325-334
pubmed: 29125244
Bull Soc Pathol Exot. 1993;86(5 Pt 2):494-9
pubmed: 7819809