DARC, Glycophorin A, Band 3, and GLUT1 Diffusion in Erythrocytes: Insights into Membrane Complexes.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
03 11 2020
03 11 2020
Historique:
received:
26
05
2020
revised:
16
09
2020
accepted:
17
09
2020
pubmed:
19
10
2020
medline:
15
5
2021
entrez:
18
10
2020
Statut:
ppublish
Résumé
Single-particle tracking offers a method to interrogate the organization of transmembrane proteins by measuring their mobilities within a cell's plasma membrane. Using this technique, the diffusion characteristics of the Duffy antigen (DARC), glycophorin A, band 3, and GLUT1 were compared under analogous conditions on intact human erythrocyte membranes. Microscopic diffusion coefficients revealed that the vast majority of all four transmembrane proteins exhibit very restricted movement but are not completely immobile. In fact, only 12% of GLUT1 resolved into a highly mobile subpopulation. Macroscopic diffusion coefficients and compartment sizes were also similar for all four proteins, with movements confined to the approximate dimensions of the "corrals" of the cortical spectrin cytoskeleton. Taken together, these data suggest that almost the entire populations of all four transmembrane proteins are immobilized by either the incorporation within large multiprotein complexes or entrapment within the protein network of the cortical spectrin cytoskeleton.
Identifiants
pubmed: 33069269
pii: S0006-3495(20)30722-0
doi: 10.1016/j.bpj.2020.09.012
pmc: PMC7677130
pii:
doi:
Substances chimiques
Anion Exchange Protein 1, Erythrocyte
0
Glucose Transporter Type 1
0
Glycophorins
0
Spectrin
12634-43-4
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1749-1759Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM024417
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM024417
Pays : United States
Informations de copyright
Copyright © 2020 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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