Prolonged use of nitric oxide donor sodium nitroprusside induces ocular hypertension in mice.
Administration, Ophthalmic
Animals
Blotting, Western
Caveolin 1
/ genetics
Drug Carriers
Immunohistochemistry
Intraocular Pressure
/ drug effects
Mice
Mice, Inbred C57BL
Mice, Knockout
Nanoparticles
Nitric Oxide Donors
/ administration & dosage
Nitroprusside
/ administration & dosage
Ocular Hypertension
/ chemically induced
Ophthalmic Solutions
Peroxynitrous Acid
/ metabolism
Tonometry, Ocular
Trabecular Meshwork
/ metabolism
Antioxidant
Intraocular hypertension
Mesoporous silica nanoparticles
Nitric oxide donor
Peroxynitrite anion
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
02
03
2020
revised:
02
09
2020
accepted:
28
09
2020
pubmed:
19
10
2020
medline:
17
4
2021
entrez:
18
10
2020
Statut:
ppublish
Résumé
Nitric oxide (NO) donors are promising therapeutic candidates for treating intraocular hypertension (IOP) and glaucoma. This study aims to investigate the effect of prolonged use of NO donor sodium nitroprusside (SNP) on IOP. Since SNP has a short biological half-life, a nanoparticle drug delivery system (mesoporous silica nanoparticles) has been used to deliver SNP to the target tissues (trabecular meshwork and Schlemm's canal). We find that the sustained use of NO donor initially reduced IOP followed, surprisingly, by IOP elevation, which could not recover by drug withdraw but could be reversed by the antioxidant MnTMPyP application. The IOP elevation and normalization coincide with increased and reduced protein nitration in the mouse conventional outflow tissue. These findings suggest that the prolonged use of NO donor SNP may be problematic as it can cause outflow tissue damage by protein nitration. MnTMPyP is protective of the nitrative damage which could be considered to be co-applied with NO donors.
Identifiants
pubmed: 33069697
pii: S0014-4835(20)30538-8
doi: 10.1016/j.exer.2020.108280
pii:
doi:
Substances chimiques
Cav1 protein, mouse
0
Caveolin 1
0
Drug Carriers
0
Nitric Oxide Donors
0
Ophthalmic Solutions
0
Peroxynitrous Acid
14691-52-2
Nitroprusside
169D1260KM
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108280Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.