Visualization of Spatially-Controlled Vasospasm by Sympathetic Nerve-Mediated ROCK Activation.

Animals Autonomic Nervous System / physiology Calcium Signaling / physiology Fluorescence Resonance Energy Transfer Mice Mice, Inbred C57BL Mice, Transgenic Muscle, Smooth, Vascular / enzymology Vasoconstriction / physiology rho-Associated Kinases / metabolism

Journal

The American journal of pathology
ISSN: 1525-2191
Titre abrégé: Am J Pathol
Pays: United States
ID NLM: 0370502

Informations de publication

Date de publication:
01 2021
Historique:
received: 03 04 2020
revised: 06 09 2020
accepted: 23 09 2020
pubmed: 19 10 2020
medline: 23 1 2021
entrez: 18 10 2020
Statut: ppublish

Résumé

Contraction of vascular smooth muscle is regulated primarily by calcium concentration and secondarily by ROCK activity within the cells. In contrast to the wealth of information regarding regulation of calcium concentration, little is known about the spatiotemporal regulation of ROCK activity in live blood vessels. Here, we report ROCK activation in subcutaneous arterioles in a transgenic mouse line that expresses a genetically encoded ROCK biosensor based on the principle of Fӧrster resonance energy transfer by two-photon excitation in vivo imaging. Rapid vasospasm was induced upon laser ablation of arterioles, concomitant with a transient increase in calcium concentration in arteriolar smooth muscles. Unlike the increase in calcium concentration, vasoconstriction and ROCK activation continued for several minutes after irradiation. Both the ROCK inhibitor, fasudil, and the ganglionic nicotinic acetylcholine receptor blocker, hexamethonium, inhibited laser-induced ROCK activation and reduced the duration of vasospasm at the segments distant from the irradiated point. These observations suggest that vasoconstriction is initially triggered by a rapid surge of cytoplasmic calcium and then maintained by sympathetic nerve-mediated ROCK activation.

Identifiants

DOI: 10.1016/j.ajpath.2020.09.012 PMID: 33069718
pubmed: 33069718
pii: S0002-9440(20)30459-4
doi: 10.1016/j.ajpath.2020.09.012
pii:
doi:

Substances chimiques

rho-Associated Kinases EC 2.7.11.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

194-203

Informations de copyright

Copyright © 2021 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

Auteurs

Ayako Imanishi (A)

Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.

Hiroshi Ichise (H)

Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.

Chuyun Fan (C)

Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.

Yasuaki Nakagawa (Y)

Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Koichiro Kuwahara (K)

Department of Cardiovascular Medicine, Shinshu University School of Medicine, Nagano, Japan.

Kenta Sumiyama (K)

Laboratory for Mouse Genetic Engineering, RIKEN Center for Biosystems Dynamics Research, Osaka, Japan.

Michiyuki Matsuda (M)

Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, Japan; Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Kenta Terai (K)

Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, Japan. Electronic address: terai.kenta.5m@kyoto-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Visualization of Spatially-Controlled...
questionsmedicales.fr › Système nerveux › Système nerveux périphérique › Système nerveux autonome › Visualization of Spatially-Controlled...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Systèmes de seconds messagers › Signalisation calcique › Visualization of Spatially-Controlled...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Transfert d'énergie par résonance de fluorescence › Visualization of Spatially-Controlled...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Visualization of Spatially-Controlled...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Visualization of Spatially-Controlled...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Visualization of Spatially-Controlled...
questionsmedicales.fr › Tissus › Muscles › Muscles lisses › Muscles lisses vasculaires › Visualization of Spatially-Controlled...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiologiques cardiovasculaires › Hémodynamique › Vasoconstriction › Visualization of Spatially-Controlled...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › rho-Associated Kinases › Visualization of Spatially-Controlled...