Bone regeneration in a mouse model of type 1 diabetes: Influence of sex, vitamin D3, and insulin.
Animals
Bone Regeneration
/ drug effects
Cholecalciferol
/ pharmacology
Diabetes Mellitus, Experimental
/ complications
Diabetes Mellitus, Type 1
/ complications
Female
Gene Expression Regulation
Insulin
/ pharmacology
Insulin-Like Growth Factor I
/ genetics
Male
Mice
Mice, Inbred C57BL
Osteoclasts
/ metabolism
Osteogenesis
/ drug effects
Sex Factors
Streptozocin
X-Ray Microtomography
Bone healing
Insulin
Mice
Sex
Type 1 diabetes
Vitamin D3
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
15 Dec 2020
15 Dec 2020
Historique:
received:
22
06
2020
revised:
01
10
2020
accepted:
07
10
2020
pubmed:
19
10
2020
medline:
16
12
2020
entrez:
18
10
2020
Statut:
ppublish
Résumé
This study set forth a question: are there any differences in bone responses to insulin and/or vitamin D3 treatment in female and male type 1 diabetic (T1D) mice? To address this issue, a non-critical sized femur defect was created in streptozotocin (STZ)-T1D mice. Control non-diabetic and T1D female and male mice received: saline; vitamin D3; insulin; or vitamin D3 plus insulin, for 21 days. Female and male T1D mice showed impaired bone healing, as indicated by histological and micro-computed tomography (micro-CT) analysis. Vitamin D3 or insulin improved the bone regeneration in T1D mice, irrespective of sex. Vitamin D3 plus insulin did not exhibit any additional effects. There were no differences regarding the numbers of TRAP-stained osteoclasts in either evaluated groups. The osteoblast-related gene osterix was upregulated in vitamin D3-treated male T1D mice, as revealed by RT-qPCR. Female T1D mice treated with vitamin D3, insulin, or vitamin D3 plus insulin presented an increased expression of insulin growth factor-1 (IGF-1) mRNA. Conversely, IGF-1 mRNA levels were reduced by the same treatments in male TD1 mice. Altogether, the results suggested that T1D similarly delayed the osseous healing in female and male mice, with beneficial effects for either vitamin D3 or insulin in T1D mice of both sexes. However, data indicated marked sex differences regarding the expression of genes implicated in bone formation, in T1D mice treated with vitamin D3 and/or insulin.
Identifiants
pubmed: 33069738
pii: S0024-3205(20)31346-1
doi: 10.1016/j.lfs.2020.118593
pii:
doi:
Substances chimiques
Insulin
0
insulin-like growth factor-1, mouse
0
Cholecalciferol
1C6V77QF41
Streptozocin
5W494URQ81
Insulin-Like Growth Factor I
67763-96-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
118593Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.