Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes.
AFM
Localisation microscopy
Multi-modal microscopy
Podosome
Super-resolution
Journal
European journal of cell biology
ISSN: 1618-1298
Titre abrégé: Eur J Cell Biol
Pays: Germany
ID NLM: 7906240
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
16
02
2020
revised:
10
06
2020
accepted:
01
07
2020
pubmed:
19
10
2020
medline:
5
8
2021
entrez:
18
10
2020
Statut:
ppublish
Résumé
Podosomes are mechanosensitive attachment/invasion structures that form on the matrix-adhesion interface of cells and protrude into the extracellular matrix to probe and remodel. Despite their central role in many cellular processes, their exact molecular structure and function remain only partially understood. We review recent progress in molecular scale imaging of podosome architecture, including our newly developed localisation microscopy technique termed HAWK which enables artefact-free live-cell super-resolution microscopy of podosome ring proteins, and report new results on combining fluorescence localisation microscopy (STORM/PALM) and atomic force microscopy (AFM) on one setup, where localisation microscopy provides the location and dynamics of fluorescently labelled podosome components, while the spatial variation of stiffness is mapped with AFM. For two-colour localisation microscopy we combine iFluor-647, which has previously been shown to eliminate the need to change buffer between imaging modes, with the photoswitchable protein mEOS3.2, which also enables live cell imaging.
Identifiants
pubmed: 33070038
pii: S0171-9335(20)30045-5
doi: 10.1016/j.ejcb.2020.151106
pmc: PMC7768945
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
151106Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier GmbH.. All rights reserved.
Références
Nat Commun. 2014 Nov 11;5:5343
pubmed: 25385672
Nat Commun. 2019 Nov 15;10(1):5171
pubmed: 31729386
Cell Rep. 2019 Dec 10;29(11):3385-3393.e6
pubmed: 31825823
PLoS One. 2014 Feb 11;9(2):e88251
pubmed: 24523880
Sci Rep. 2020 Jan 24;10(1):1122
pubmed: 31980680
Nano Lett. 2015 Aug 12;15(8):4896-904
pubmed: 26121585
J Cell Physiol. 1992 Oct;153(1):30-7
pubmed: 1522133
Sci Rep. 2015 Jan 21;5:7924
pubmed: 25603780
Nat Methods. 2012 May 13;9(7):727-9
pubmed: 22581370
Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):21016-21
pubmed: 21081699
J Cell Biol. 1984 Nov;99(5):1696-705
pubmed: 6436255
PLoS One. 2013 Jun 17;8(6):e66608
pubmed: 23799123
Philos Trans R Soc Lond B Biol Sci. 2019 Aug 19;374(1779):20180228
pubmed: 31431172
Nat Methods. 2018 Sep;15(9):689-692
pubmed: 30061677
Nat Commun. 2017 Jan 12;8:13558
pubmed: 28079054
Nat Commun. 2018 Feb 6;9(1):515
pubmed: 29410425
PLoS One. 2007 Jan 31;2(1):e179
pubmed: 17264882
Chem Rev. 2017 Jun 14;117(11):7428-7456
pubmed: 28045508
Histochem Cell Biol. 2015 Aug;144(2):123-31
pubmed: 26138928
Immunol Rev. 2014 Nov;262(1):216-31
pubmed: 25319337
J Cell Sci. 2010 May 1;123(Pt 9):1427-37
pubmed: 20356925
Methods Appl Fluoresc. 2018 Jul 09;6(4):045002
pubmed: 29956675
Front Immunol. 2018 Aug 22;9:1908
pubmed: 30186284
Nat Protoc. 2011 Jun 16;6(7):991-1009
pubmed: 21720313
Eur J Cell Biol. 2014 Oct;93(10-12):380-7
pubmed: 25454791
Nat Methods. 2015 Aug;12(8):717-24
pubmed: 26076424
J Biol Methods. 2014;1(2):
pubmed: 25606571
Methods Mol Biol. 2017;1663:105-113
pubmed: 28924662
J Cell Sci. 2015 Jan 15;128(2):251-65
pubmed: 25413351
Chemphyschem. 2014 Mar 17;15(4):647-50
pubmed: 24273067
Cytoskeleton (Hoboken). 2013 Nov;70(11):729-40
pubmed: 24027190
Bioinformatics. 2014 Aug 15;30(16):2389-90
pubmed: 24771516
Nat Methods. 2013 Jun;10(6):557-62
pubmed: 23624665
Sensors (Basel). 2017 Apr 24;17(4):
pubmed: 28441775
ACS Nano. 2017 Apr 25;11(4):4028-4040
pubmed: 28355484
Biochem J. 2016 Oct 15;473(20):3385-3399
pubmed: 27729584
Small. 2017 May;13(17):
pubmed: 28257567
Angew Chem Int Ed Engl. 2008;47(33):6172-6
pubmed: 18646237
Methods. 2017 Feb 15;115:9-16
pubmed: 27840289
Mol Biol Cell. 2003 Feb;14(2):407-16
pubmed: 12589043
J Cell Biol. 2011 Mar 7;192(5):883-97
pubmed: 21357746
Nat Nanotechnol. 2008 May;3(5):261-9
pubmed: 18654521
J Cell Sci. 2001 Aug;114(Pt 15):2775-86
pubmed: 11683411
Nat Mater. 2019 Jun;18(6):638-649
pubmed: 31114072
Rep Prog Phys. 2018 Feb;81(2):024601
pubmed: 28869217
Mol Biol Cell. 2013 Jul;24(13):2112-23
pubmed: 23637461
FEBS J. 2018 Jan;285(1):8-27
pubmed: 28548369
Science. 2006 Sep 15;313(5793):1642-5
pubmed: 16902090
Phys Rev Lett. 1986 Mar 3;56(9):930-933
pubmed: 10033323
Nat Methods. 2006 Oct;3(10):793-5
pubmed: 16896339
J Cell Biol. 2017 Jan 2;216(1):181-197
pubmed: 28007915
Nat Methods. 2011 Dec 04;9(2):195-200
pubmed: 22138825
Nat Commun. 2016 Aug 12;7:12471
pubmed: 27514992
Nat Methods. 2006 May;3(5):347-55
pubmed: 16628204