Genetic Tools to Study Cardiovascular Biology.
Cre
barcoding
cardiovascular
clonal analysis
genetic tools
imaging
mosaics
transgenics
Journal
Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006
Informations de publication
Date de publication:
2020
2020
Historique:
received:
25
06
2020
accepted:
06
08
2020
entrez:
19
10
2020
pubmed:
20
10
2020
medline:
20
10
2020
Statut:
epublish
Résumé
Progress in biomedical science is tightly associated with the improvement of methods and genetic tools to manipulate and analyze gene function in mice, the most widely used model organism in biomedical research. The joint effort of numerous individual laboratories and consortiums has contributed to the creation of a large genetic resource that enables scientists to image cells, probe signaling pathways activities, or modify a gene function in any desired cell type or time point, à la carte. However, as these tools significantly increase in number and become more sophisticated, it is more difficult to keep track of each tool's possibilities and understand their advantages and disadvantages. Knowing the best currently available genetic technology to answer a particular biological question is key to reach a higher standard in biomedical research. In this review, we list and discuss the main advantages and disadvantages of available mammalian genetic technology to analyze cardiovascular cell biology at higher cellular and molecular resolution. We start with the most simple and classical genetic approaches and end with the most advanced technology available to fluorescently label cells, conditionally target their genes, image their clonal expansion, and decode their lineages.
Identifiants
pubmed: 33071802
doi: 10.3389/fphys.2020.01084
pmc: PMC7541935
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
1084Subventions
Organisme : Austrian Science Fund FWF
ID : J 4358
Pays : Austria
Informations de copyright
Copyright © 2020 Garcia-Gonzalez, Mühleder, Fernández-Chacón and Benedito.
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