Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney.
glomerulus
kidney
mass spectrometry
proteomics
single cell analysis
Journal
Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047
Informations de publication
Date de publication:
2020
2020
Historique:
received:
25
11
2019
accepted:
21
07
2020
entrez:
19
10
2020
pubmed:
20
10
2020
medline:
20
10
2020
Statut:
epublish
Résumé
Molecular assessments at the single cell level can accelerate biological research by providing detailed assessments of cellular organization and tissue heterogeneity in both disease and health. The human kidney has complex multi-cellular states with varying functionality, much of which can now be completely harnessed with recent technological advances in tissue proteomics at a near single-cell level. We discuss the foundational steps in the first application of this mass spectrometry (MS) based proteomics method for analysis of sub-sections of the normal human kidney, as part of the Kidney Precision Medicine Project (KPMP). Using ~30-40 laser captured micro-dissected kidney cells, we identified more than 2,500 human proteins, with specificity to the proximal tubular (PT;
Identifiants
pubmed: 33072769
doi: 10.3389/fmed.2020.00499
pmc: PMC7533534
doi:
Types de publication
Journal Article
Langues
eng
Pagination
499Subventions
Organisme : NIDDK NIH HHS
ID : DP3 DK110844
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK114886
Pays : United States
Organisme : NIDDK NIH HHS
ID : U2C DK114886
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 Sigdel, Piehowski, Roy, Liberto, Hansen, Swensen, Zhao, Zhu, Rashmi, Schroeder, Damm, Sur, Luo, Yang, Qian, Sarwal and the Kidney Precision Medicine Project (KPMP) Consortium.
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