NADPH-cytochrome P450 reductase mediates the susceptibility of Asian citrus psyllid Diaphorina citri to imidacloprid and thiamethoxam.
Diaphorina citri
NADPH-cytochrome P450 reductase
RNA interference
eukaryotic expression
imidacloprid
thiamethoxam
Journal
Pest management science
ISSN: 1526-4998
Titre abrégé: Pest Manag Sci
Pays: England
ID NLM: 100898744
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
06
08
2020
revised:
14
10
2020
accepted:
19
10
2020
pubmed:
20
10
2020
medline:
15
1
2021
entrez:
19
10
2020
Statut:
ppublish
Résumé
The Asian citrus psyllid Diaphorina citri has developed high levels of resistance to many insecticides, and understanding its resistance mechanism will aid in the chemical control of this species. Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is crucial in cytochrome P450 function, and in some insects CPR knockdown has increased their susceptibility to insecticides. However, the CPR from D. citri has not been characterized and its function is undescribed. The CPR gene of D. citri (DcCPR) was cloned and sequenced. The expression level of DcCPR, determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analysis, was highest in the midgut and in nymphs. After feeding on double-stranded RNA for 72 h, the DcCPR messenger RNA level in D. citri adults decreased by 68.4%, and the susceptibility of D. citri to imidacloprid and thiamethoxam significantly increased. Meanwhile, after DcCPR silencing, the specific activities of DcCPR protein and P450s were significantly reduced by 41.6% and 44.7%, respectively. The subsequent western blot analysis and quantification of band intensity also showed that DcCPR content significantly decreased, consistent with the results of the specific activity test. In a eukaryotic expression assay, the viability of cells expressing DcCPR was significantly higher than the viability of cells expressing green fluorescent protein (GFP) when cells were exposed to imidacloprid or thiamethoxam. These results indicate that DcCPR contributes to D. citri susceptibility to imidacloprid and thiamethoxam.
Sections du résumé
BACKGROUND
BACKGROUND
The Asian citrus psyllid Diaphorina citri has developed high levels of resistance to many insecticides, and understanding its resistance mechanism will aid in the chemical control of this species. Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is crucial in cytochrome P450 function, and in some insects CPR knockdown has increased their susceptibility to insecticides. However, the CPR from D. citri has not been characterized and its function is undescribed.
RESULTS
RESULTS
The CPR gene of D. citri (DcCPR) was cloned and sequenced. The expression level of DcCPR, determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analysis, was highest in the midgut and in nymphs. After feeding on double-stranded RNA for 72 h, the DcCPR messenger RNA level in D. citri adults decreased by 68.4%, and the susceptibility of D. citri to imidacloprid and thiamethoxam significantly increased. Meanwhile, after DcCPR silencing, the specific activities of DcCPR protein and P450s were significantly reduced by 41.6% and 44.7%, respectively. The subsequent western blot analysis and quantification of band intensity also showed that DcCPR content significantly decreased, consistent with the results of the specific activity test. In a eukaryotic expression assay, the viability of cells expressing DcCPR was significantly higher than the viability of cells expressing green fluorescent protein (GFP) when cells were exposed to imidacloprid or thiamethoxam.
CONCLUSION
CONCLUSIONS
These results indicate that DcCPR contributes to D. citri susceptibility to imidacloprid and thiamethoxam.
Substances chimiques
Insecticides
0
Neonicotinoids
0
Nitro Compounds
0
imidacloprid
3BN7M937V8
Thiamethoxam
747IC8B487
Cytochrome P-450 Enzyme System
9035-51-2
NADPH-Ferrihemoprotein Reductase
EC 1.6.2.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
677-685Subventions
Organisme : earmarked fund for the Modern Agroindustry (Citrus) Technology Research System of China
ID : CARS-26
Organisme : National Key Research and Development Program
ID : 2018YFD0201508
Organisme : Special Foundation for National Science and Technology Basic Research Program of China
ID : 2018FY101105
Organisme : Special Project for Performance Incentive and Guidance of Scientific Research Institutions in Chongqing
ID : cstc2018jxjl80038
Informations de copyright
© 2020 Society of Chemical Industry.
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