Discovery of cell-internalizing artificial nucleic acid aptamers for lung fibroblasts and targeted drug delivery.
Aptamers, Nucleotide
/ chemistry
Base Sequence
Cell Line
Cell Proliferation
/ drug effects
DNA
/ chemistry
Drug Carriers
/ chemistry
Fibroblasts
/ cytology
Gene Library
Humans
Idiopathic Pulmonary Fibrosis
/ drug therapy
Indoles
/ chemistry
Ligands
Lung
Nucleic Acids
/ chemistry
Oligopeptides
/ chemistry
Artificial nucleic acid aptamer
Drug conjugate
Internalization
Lung fibroblast
SELEX
Journal
Bioorganic chemistry
ISSN: 1090-2120
Titre abrégé: Bioorg Chem
Pays: United States
ID NLM: 1303703
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
01
09
2020
accepted:
24
09
2020
pubmed:
20
10
2020
medline:
20
3
2021
entrez:
19
10
2020
Statut:
ppublish
Résumé
Lung fibroblasts play major roles in the lung repair/fibrosis process through synthesis and remodeling of extracellular matrix. Those aberrant activations and elevated proliferations are associated with several fibrotic lung diseases, such as idiopathic pulmonary fibrosis (IPF). Targeting fibroblasts is a promising approach for preventing aberrant remodeling of lung architecture and protect irreversible pulmonary fibrosis. In this study, we developed an aptamer that can target lung fibroblasts and explored its potential as a delivery vehicle of cytotoxic agents intracellularly. The aptamer was discovered from artificial nucleic acid libraries through cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX). This indole-modified aptamer can bind to LL97A cells, a fibroblast cell line derived from IPF patients, with high affinity (K
Identifiants
pubmed: 33074117
pii: S0045-2068(20)31619-9
doi: 10.1016/j.bioorg.2020.104321
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Drug Carriers
0
Indoles
0
Ligands
0
Nucleic Acids
0
Oligopeptides
0
monomethylauristatin F
0
indole
8724FJW4M5
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104321Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.