Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization.
Biotinylation
Blotting
Cell surface
Egg activation
Fertilization
Signal transduction
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
19
10
2020
pubmed:
20
10
2020
medline:
2
4
2021
Statut:
ppublish
Résumé
To provide a better understanding of the composition of the egg cell membrane, we describe a method in which proteins and peptides that are either naturally released by the egg or cleaved by sperm proteases can be collected, analyzed, and identified. Such molecules are captured and isolated from the surrounding seawater via biotinylation, before being concentrated by an affinity interaction and subsequently analyzed by western blotting and mass spectrometry.
Identifiants
pubmed: 33074539
doi: 10.1007/978-1-0716-0974-3_9
doi:
Substances chimiques
Egg Proteins
0
Membrane Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
151-161Subventions
Organisme : NICHD NIH HHS
ID : R15 HD094324
Pays : United States
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