Cholesterol Loaded Cyclodextrin Supplementation Enhances the Cholesterol-to-Phospholipid Ratio and Diminishes Oxidative Stress in Jack Spermatozoa During Cryopreservation.
CLC
Cryopreservation
HOST
Jacks
Malondialdehyde
Oxidative stress
Journal
Journal of equine veterinary science
ISSN: 0737-0806
Titre abrégé: J Equine Vet Sci
Pays: United States
ID NLM: 8216840
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
08
06
2020
revised:
24
08
2020
accepted:
24
08
2020
entrez:
20
10
2020
pubmed:
21
10
2020
medline:
23
3
2021
Statut:
ppublish
Résumé
The present study was conducted with the hypothesis that addition of cholesterol to the extender would stabilize the sperm membranes by increasing the cholesterol-to-phospholipid (C:P) ratio and would result in an improved post-thaw semen quality and reduce oxidative stress in the jack (Martina franca) semen. Forty-eight ejaculates from six jacks were collected and analyzed for the present study. The freshly collected semen sample of each jack stallion was divided into five equal fractions after addition of the primary extender without cholesterol-loaded cyclodextrin (CLC) (C) and with 1, 1.5, 2, and 3 mg/mL CLC to obtain 120 × 10
Identifiants
pubmed: 33077064
pii: S0737-0806(20)30328-2
doi: 10.1016/j.jevs.2020.103237
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Cyclodextrins
0
Phospholipids
0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103237Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.