Mechanism by which the combination of SjCL3 and SjGAPDH protects against Schistosoma japonicum infection.
Animals
Cathepsins
/ administration & dosage
Female
Glyceraldehyde-3-Phosphate Dehydrogenases
/ administration & dosage
Helminth Proteins
/ administration & dosage
Mice
Mice, Inbred BALB C
Schistosoma japonicum
/ immunology
Schistosomiasis japonica
/ prevention & control
T-Lymphocytes, Regulatory
/ immunology
Vaccination
Vaccines
/ administration & dosage
Cathepsin L3
GAPDH
Immunoprotection
Schistosoma japonicum
Tregs
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
24
01
2020
accepted:
04
10
2020
pubmed:
21
10
2020
medline:
13
3
2021
entrez:
20
10
2020
Statut:
ppublish
Résumé
A vaccine is an important method to control schistosomiasis. Molecules related to lung-stage schistosomulum are considered potential vaccine candidates. We previously showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and cathepsin L3 (CL3) displayed differential expression in the lung-stage schistosomula of Schistosoma japonicum cocultured with host cells. In the present study, we prepared the two proteins and detected the protective effects of SjGAPDH by immunizing mice with this protein alone and in combination with SjCL3 with or without Freund's adjuvant. Then, we investigated the possible mechanisms underlying S. japonicum infection. The results showed that vaccination of adjuvanted SjGAPDH decreased the worm burden (37.8%) and egg load (38.1%), and the combination of adjuvanted SjGAPDH and SjCL3 further decreased the worm burden (65.6%) and egg load (70.9%) during Schistosoma japonicum infection. However, the immunization of a combination of adjuvant-free SjGAPDH and SjCL3 displayed a lower protective effect (< 15%) than those of the adjuvanted SjCL3, the adjuvanted SjGAPDH, and a combination of adjuvanted SjGAPDH and SjCL3. Flow cytometric results showed that the frequency of regulatory T cells (Tregs) was lower (P < 0.05) in the group with adjuvanted SjGAPDH and SjCL3 (2.61%) than the remaining groups. The enzyme-linked immunosorbent assay (ELISA) results indicated that except for the uninfected and infected control groups, the remaining groups displayed a Th1-type shift in immune responses. These results showed the immunization of SjGAPDH resulted in partial protection (approximately 38%); inoculation with a combination of SjCL3 and SjGAPDH in Freund's adjuvant resulted in a high immunoprotective effect (> 65%) against Schistosoma japonicum infection in mice, which was possibly caused by the reduced percentage of Tregs and a Th1-type shift in immune responses; and SjCL3 has no adjuvant-like effect, dissimilar to SmCL3.
Identifiants
pubmed: 33079271
doi: 10.1007/s00436-020-06916-9
pii: 10.1007/s00436-020-06916-9
doi:
Substances chimiques
Helminth Proteins
0
Vaccines
0
Glyceraldehyde-3-Phosphate Dehydrogenases
EC 1.2.1.-
Cathepsins
EC 3.4.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
173-185Subventions
Organisme : Scientific Research Subject of the Health and Family Planning Commission of Hubei province
ID : XF2012-18
Organisme : National Natural Science Foundation of China
ID : 81273010
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