Highly sensitive detection of Campylobacter spp. In chicken meat using a silica nanoparticle enhanced dot blot DNA biosensor.
Campylobacter
DNA dot Blot
Food safety
Multiplex bacterial detection
Si-nanoparticles
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
01 Jan 2021
01 Jan 2021
Historique:
received:
17
08
2020
revised:
21
09
2020
accepted:
04
10
2020
pubmed:
21
10
2020
medline:
1
5
2021
entrez:
20
10
2020
Statut:
ppublish
Résumé
Paper-based DNA biosensors are powerful tools in point-of-care diagnostics since they are affordable, portable, user-friendly, rapid and robust. However, their sensitivity is not always as high as required to enable DNA quantification. To improve the response of standard dot blots, we have applied a new enhancement strategy that increases the sensitivity of assays based on the use of biotinylated silica-nanoparticles (biotin-Si-NPs). After immobilization of a genomic Campylobacter DNA onto a paper membrane, and addition of a biotinylated-DNA detection probe, hybridization was evidenced using streptavidin-conjugated to horseradish peroxidase (HRP) in the presence of luminol and H
Identifiants
pubmed: 33080463
pii: S0956-5663(20)30678-3
doi: 10.1016/j.bios.2020.112689
pii:
doi:
Substances chimiques
Silicon Dioxide
7631-86-9
DNA
9007-49-2
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112689Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.