Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy.
Imaging and sensing
Microscopy
Journal
Light, science & applications
ISSN: 2047-7538
Titre abrégé: Light Sci Appl
Pays: England
ID NLM: 101610753
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
05
2020
revised:
01
09
2020
accepted:
04
09
2020
entrez:
21
10
2020
pubmed:
22
10
2020
medline:
22
10
2020
Statut:
epublish
Résumé
Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth range. Here, we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective's aperture stop. Placing the phase mask at the aperture stop significantly reduces the size of the device, and varying the focal lengths enables a uniform resolution across a wide depth range. The phase mask encodes the 3D fluorescence intensity into a single 2D measurement, and the 3D volume is recovered by solving a sparsity-constrained inverse problem. We provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the field-varying aberrations in miniature objectives. We demonstrate a prototype that is 17 mm tall and weighs 2.5 grams, achieving 2.76 μm lateral, and 15 μm axial resolution across most of the 900 × 700 × 390 μm
Identifiants
pubmed: 33082940
doi: 10.1038/s41377-020-00403-7
pii: 10.1038/s41377-020-00403-7
pmc: PMC7532148
doi:
Types de publication
Journal Article
Langues
eng
Pagination
171Subventions
Organisme : NEI NIH HHS
ID : R21 EY027597
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare that they have no conflict of interest.
Références
Neuron. 2001 Sep 27;31(6):903-12
pubmed: 11580892
Opt Express. 2020 Mar 16;28(6):8384-8399
pubmed: 32225465
IEEE Trans Image Process. 2013 Feb;22(2):447-58
pubmed: 22955907
J Opt Soc Am A Opt Image Sci Vis. 2005 Mar;22(3):504-13
pubmed: 15770988
Sci Adv. 2019 Dec 06;5(12):eaaw5595
pubmed: 31840055
Opt Lett. 2010 May 1;35(9):1413-5
pubmed: 20436587
Sci Adv. 2017 Feb 15;3(2):e1602655
pubmed: 28246646
Curr Protoc Neurosci. 2018 Jul;84(1):e51
pubmed: 29944206
Elife. 2020 Jan 14;9:
pubmed: 31934857
J Neural Eng. 2017 Aug;14(4):045001
pubmed: 28514229
J Neurosci Methods. 2017 Nov 1;291:83-94
pubmed: 28782629
Opt Express. 2008 Dec 22;16(26):22048-57
pubmed: 19104639
Opt Express. 2013 Oct 21;21(21):25418-39
pubmed: 24150383
Nat Methods. 2017 Jul;14(7):713-719
pubmed: 28553965
Sci Adv. 2017 Dec 08;3(12):e1701548
pubmed: 29226243
IEEE Trans Image Process. 2017 Feb;26(2):539-548
pubmed: 27875224
Nat Methods. 2018 Jun;15(6):429-432
pubmed: 29736000
Opt Express. 2019 Sep 2;27(18):25573-25594
pubmed: 31510428
Nat Methods. 2017 Aug;14(8):811-818
pubmed: 28650477
Nat Methods. 2011 Sep 11;8(10):871-8
pubmed: 21909102
Biomed Opt Express. 2017 Dec 22;9(1):335-346
pubmed: 29359107
Opt Express. 2016 Sep 5;24(18):20792-8
pubmed: 27607682