Evidence of a non-apoptotic mode of cell death in microglial BV-2 cells exposed to different concentrations of zinc oxide nanoparticles.
Lysosomal destabilisation
Microglial BV-2 cells
Mitochondrial dysfunction
Non-apoptotic mode of cell death
Oxidative stress
Plasma membrane permeability
Zinc oxide nanoparticles (ZnO NPs)
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
17
04
2020
accepted:
04
10
2020
pubmed:
22
10
2020
medline:
5
3
2021
entrez:
21
10
2020
Statut:
ppublish
Résumé
Zinc oxide nanoparticles (ZnO NPs) possess huge application potential. However, the toxicity of ZnO NPs is a great cause of concern. Indeed, ZnO NPs have been found to cause neurotoxicity. As microglial dysfunctions have been linked to the neurotoxic potential of NPs, the physico-chemical properties of ZnO NPs were determined and their cytotoxic effects were characterised on murine microglial BV-2 cells. In-house prepared and meticulously characterised ZnO NPs exhibited narrow size distribution with an average size of around 20 nm and a zeta potential at physiological pH around 24 mV. ZnO NPs did not exhibit aggregation in the cell culture medium. When microglial BV-2 cells were exposed for 6 and 24 h to ZnO NPs (5, 10, 20, 40, and 80 μg/mL), several cell damages were observed. Cellular accumulation of NPs in microglial BV-2 cells was associated with cell growth inhibition and cell death induction, measured by the trypan blue exclusion and MTT assays. Mitochondrial dysfunction and lysosomal alteration were associated with increased plasma membrane permeability measured by staining with DiOC
Identifiants
pubmed: 33083954
doi: 10.1007/s11356-020-11100-8
pii: 10.1007/s11356-020-11100-8
doi:
Substances chimiques
Reactive Oxygen Species
0
Zinc Oxide
SOI2LOH54Z
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12500-12520Références
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