Line-field confocal optical coherence tomography-Practical applications in dermatology and comparison with established imaging methods.
dermatology
line-field confocal OCT
optical coherence tomography
reflectance confocal microscopy
skin cancer
skin imaging
Journal
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
ISSN: 1600-0846
Titre abrégé: Skin Res Technol
Pays: England
ID NLM: 9504453
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
16
06
2020
accepted:
07
09
2020
pubmed:
22
10
2020
medline:
19
8
2021
entrez:
21
10
2020
Statut:
ppublish
Résumé
Non-invasive diagnostic techniques in dermatology gained increasing popularity in the last decade. Reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) are meanwhile established in research and clinical routine. While OCT is mainly indicated for detecting non-melanoma skin cancer, RCM has proven its usefulness additionally in distinguishing melanocytic lesions. Line-field confocal optical coherence tomography (LC-OCT) is an emerging tool combining the principles of both above-mentioned methods. Healthy skin at different body sites and exemplary skin lesions (basal cell carcinoma, malignant melanoma, actinic keratosis) were examined using dermoscopy, RCM, OCT and LC-OCT. Standard features for RCM and OCT and comparable features for LC-OCT were analysed. LC-OCT has a lower penetration depth but superior resolution compared to OCT. In comparison with RCM, which provides only horizontal sections, LC-OCT creates both vertical and horizontal images in real time and has nearly the same cellular resolution. Our preliminary experiences suggest that LC-OCT combines the advantages of RCM and OCT, with optimal resolution and penetration depth to diagnose all types of skin cancer. Larger systematic studies are needed to further characterize the field of use of this device and its sensitivity and specificity compared to histology.
Sections du résumé
BACKGROUND
BACKGROUND
Non-invasive diagnostic techniques in dermatology gained increasing popularity in the last decade. Reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) are meanwhile established in research and clinical routine. While OCT is mainly indicated for detecting non-melanoma skin cancer, RCM has proven its usefulness additionally in distinguishing melanocytic lesions. Line-field confocal optical coherence tomography (LC-OCT) is an emerging tool combining the principles of both above-mentioned methods.
METHODS
METHODS
Healthy skin at different body sites and exemplary skin lesions (basal cell carcinoma, malignant melanoma, actinic keratosis) were examined using dermoscopy, RCM, OCT and LC-OCT. Standard features for RCM and OCT and comparable features for LC-OCT were analysed.
RESULTS
RESULTS
LC-OCT has a lower penetration depth but superior resolution compared to OCT. In comparison with RCM, which provides only horizontal sections, LC-OCT creates both vertical and horizontal images in real time and has nearly the same cellular resolution.
DISCUSSION
CONCLUSIONS
Our preliminary experiences suggest that LC-OCT combines the advantages of RCM and OCT, with optimal resolution and penetration depth to diagnose all types of skin cancer. Larger systematic studies are needed to further characterize the field of use of this device and its sensitivity and specificity compared to histology.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
340-352Subventions
Organisme : FöFoLe (Förderprogramm für Forschung und Lehre) Grant of the Ludwig Maximilian University of Munich
ID : Grant Number 1022-2018
Informations de copyright
© 2020 The Authors. Skin Research and Technology published by John Wiley & Sons Ltd.
Références
Holmes J., von Braunmühl T., Berking C., et al. Optical coherence tomography of basal cell carcinoma: influence of location, subtype, observer variability and image quality on diagnostic performance. Br J Dermatol. 2018;178(5):1102-1110.
Ulrich M., Maier T., Kurzen H., et al. The sensitivity and specificity of optical coherence tomography for the assisted diagnosis of non-pigmented basal cell carcinoma - an observational study. Br J Dermatol. 2015;173(2):428-435.
Ferrante di Ruffano L., Dinnes J., Deeks J.J., et al. Optical coherence tomography for diagnosing skin cancer in adults. Cochrane Database Syst Rev. 2018;12:CD013189. https://doi.org/10.1002/14651858.CD013189
Welzel J., Schuh S.. Optical coherence tomography for skin pathologies. Ophthalmologe. 2018;115(6):524-527.
Schuh S., Holmes J., Ulrich M., et al. Imaging blood vessel morphology in skin: dynamic optical coherence tomography as a novel potential diagnostic tool in dermatology. Dermatol Ther (Heidelb). 2017;7(2):187-202.
Dinnes J., Deeks J.J., Chuchu N., et al. Reflectance confocal microscopy for diagnosing keratinocyte skin cancers in adults. Cochrane Database Syst Rev. 2018;12:CD013191. https://doi.org/10.1002/14651858.CD013191
Que S.K., Grant-Kels J.M., Longo C., Pellacani G.. Basics of confocal microscopy and the complexity of diagnosing skin tumors: new imaging tools in clinical practice, diagnostic workflows, cost-estimate, and new trends. Dermatol Clin. 2016;34(4):367-375.
Davis A., Levecq O., Azimani H., Siret D., Dubois A.. Simultaneous dual-band line-field confocal optical coherence tomography: application to skin imaging. Biomed Opt Express. 2019;10(2):694-706.
Dubois A., Levecq O., Azimani H., et al. Line-field confocal time-domain optical coherence tomography with dynamic focusing. Opt Express. 2018;26(26):33534-33542.
Ogien J., Levecq O., Azimani H., Dubois A.. Dual-mode line-field confocal optical coherence tomography for ultrahigh-resolution vertical and horizontal section imaging of human skin in vivo. Biomed Opt Express. 2020;11(3):1327-1335.
Dubois A., Levecq O., Azimani H., et al. Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors. J Biomed Opt. 2018;23(10):1-9.
Pedrazzani M., Breugnot J., Rouaud-Tinguely P., et al. Comparison of line-field confocal optical coherence tomography images with histological sections: Validation of a new method for in vivo and non-invasive quantification of superficial dermis thickness. Skin Res Technol. 2020;26(3):398-404. https://doi.org/10.1111/srt.12815
Rajadhyaksha M., Grossman M., Esterowitz D., Webb R.H., Anderson R.R.. In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast. J Invest Dermatol. 1995;104(6):946-952.
Que S.K., Fraga-Braghiroli N., Grant-Kels J.M., Rabinovitz H.S., Oliviero M., Scope A.. Through the looking glass: basics and principles of reflectance confocal microscopy. J Am Acad Dermatol. 2015;73(2):276-284.