Expression of huntingtin-associated protein 1 in adult mouse dorsal root ganglia and its neurochemical characterization in reference to sensory neuron subpopulations.
CB, calbindin
CGRP, calcitonin gene-related peptide
CR, calretinin
DAB, diaminobenzidine
DRG, dorsal root ganglia
HAP1, Huntingtin-associated protein 1
Huntingtin-associated protein 1
Iba1, ionized calcium-binding adapter molecule 1
Immunohistochemistry
LTMRs, low-threshold mechanoreceptors
MRGPR, Mas-related G-protein-coupled receptor
NDS, normal donkey serum
NOS, nitric oxide synthetase
NeuN, neuronal nuclei
Neurodegeneration
Neuroprotection
PB, phosphate buffer
PV, parvalbumin
Peripheral nervous system
SBMA, spinal and bulbar muscular atrophy
SP, substance P
STB, stigmoid body
Sensory neurons
TBST, Tris-buffered saline with 0.1 % Tween
TH, tyrosine hydroxylase
TRPV1, transient receptor potential vanilloid 1
VGLUT, vesicular glutamate transporter
htt, huntingtin
polyQ, polyglutamine
Journal
IBRO reports
ISSN: 2451-8301
Titre abrégé: IBRO Rep
Pays: England
ID NLM: 101691215
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
28
06
2020
accepted:
02
10
2020
entrez:
22
10
2020
pubmed:
23
10
2020
medline:
23
10
2020
Statut:
epublish
Résumé
Huntingtin-associated protein 1 (HAP1) is a polyglutamine (polyQ) length-dependent interactor with causal agents in several neurodegenerative diseases and has been regarded as a protective factor against neurodegeneration. In normal rodent brain and spinal cord, HAP1 is abundantly expressed in the areas that are spared from neurodegeneration while those areas with little HAP1 are frequent targets of neurodegeneration. We have recently showed that HAP1 is highly expressed in the spinal dorsal horn and may participate in modification/protection of certain sensory functions. Neurons in the dorsal root ganglia (DRG) transmits sensory stimuli from periphery to spinal cord/brain stem. Nevertheless, to date HAP1 expression in DRG remains unreported. In this study, the expression of HAP1 in cervical, thoracic, lumbar and sacral DRG in adult male mice and its relationships with different chemical markers for sensory neurons were examined using Western blot and immunohistochemistry. HAP1-immunoreactivity was detected in the cytoplasm of DRG neurons, and the percentage of HAP1-immunoreactive (ir) DRG neurons was ranged between 28-31 %. HAP1-immunoreactivity was comparatively more in the small cells (47-58 %) and medium cells (40-44 %) than that in the large cells (9-11 %). Double-immunostaining for HAP1 and markers for nociceptive or mechanoreceptive neurons showed that about 70-80 % of CGRP-, SP-, CB-, NOS-, TRPV1-, CR- and PV-ir neurons expressed HAP1. In contrast, HAP1 was completely lacking in TH-ir neurons. Our current study is the first to clarify that HAP1 is highly expressed in nociceptive/proprioceptive neurons but absent in light-touch-sensitive TH neurons, suggesting the potential importance of HAP1 in pain transduction and proprioception.
Identifiants
pubmed: 33089002
doi: 10.1016/j.ibror.2020.10.001
pii: S2451-8301(20)30042-X
pmc: PMC7560692
doi:
Types de publication
Journal Article
Langues
eng
Pagination
258-269Informations de copyright
© 2020 The Author(s).
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