Trypsin-Free Cultivation of 3D Mini-Tissues in an Adaptive Membrane Bioreactor.
3D tissues
cell adhesion
hollow fiber membrane bioreactors
scaffold-free tissues
temperature-responsive microgels
Journal
Advanced biosystems
ISSN: 2366-7478
Titre abrégé: Adv Biosyst
Pays: Germany
ID NLM: 101711718
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
23
03
2020
revised:
11
08
2020
pubmed:
23
10
2020
medline:
12
10
2021
entrez:
22
10
2020
Statut:
ppublish
Résumé
The production of large scaffold-free tissues is a key challenge in regenerative medicine. Nowadays, temperature-responsive polymers allow intact tissue harvesting without needing proteolytic enzymes. This method is limited to tissue culture plastic with limited upscaling capacity and plain process control. Here, a thermoresponsive hollow fiber membrane bioreactor is presented to produce large scaffold-free tissues. Intact tissues, rich in cell-to-cell connections and ECM, are harvested from a poly(N-vinylcaprolactam) microgel functionalized poly(ether sulfone)/poly(vinylpyrrolidone) hollow fiber membrane by a temperature shift. The harvested 3D tissues adhere in successive cultivation and exhibit high vitality for several days. The facile adsorptive coating waives the need for extensive surface treatment. The research is anticipated to be a starting point for upscaling the production of interconnected tissues enabling new opportunities in regenerative medicine, large-scale drug screening on physiological relevant tissues, and potentially opening new chances in cell-based therapies.
Identifiants
pubmed: 33089652
doi: 10.1002/adbi.202000081
doi:
Substances chimiques
Membranes, Artificial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000081Informations de copyright
© 2020 The Authors. Advanced Biosystems published by Wiley-VCH GmbH.
Références
K. Nishida, M. Yamato, Y. Hayashida, K. Watanabe, K. Yamamoto, E. Adachi, S. Nagai, A. Kikuchi, N. Maeda, H. Watanabe, T. Okano, Y. Tano, N. Engl. J. Med. 2004, 351, 1187.
K. Sakaguchi, T. Shimizu, T. Okano, J. Controlled Release 2015, 205, 83.
Y. Shiroyanagi, M. Yamato, Y. Yamazaki, H. Toma, T. Okano, BJU Int. 2004, 93, 1069.
C. Norotte, F. S. Marga, L. E. Niklason, G. Forgacs, Biomaterials 2009, 30, 5910.
L. G. Griffith, M. A. Swartz, Nat. Rev. Mol. Cell Biol. 2006, 7, 211.
F. Pampaloni, E. G. Reynaud, E. H. K. Stelzer, Nat. Rev. Mol. Cell Biol. 2007, 8, 839.
V. Mironov, R. P. Visconti, V. Kasyanov, G. Forgacs, C. J. Drake, R. R. Markwald, Biomaterials 2009, 30, 2164.
T. Takezawa, M. Yamazaki, Y. Mori, T. Yonaha, K. Yoshizato, J. Cell Sci. 1992, 101, 495.
N. Yamada, T. Okano, H. Sakai, F. Karikusa, Y. Sawasaki, Y. Sakurai, Makromol. Chem., Rapid Commun. 1990, 11, 571.
R. M. Sutherland, Science 1988, 240, 177.
R. M. Sutherland, J. A. McCredie, W. R. Inch, J. Natl. Cancer Inst. 1971, 46, 113.
F. Hirschhaeuser, H. Menne, C. Dittfeld, J. West, W. Mueller-Klieser, L. A. Kunz-Schughart, J. Biotechnol. 2010, 148, 3.
K. Nagase, J. Kobayashi, T. Okano, J. R. Soc., Interface 2009, 6, S293.
A. Tamura, J. Kobayashi, M. Yamato, T. Okano, Biomaterials 2012, 33, 3803.
M. Karg, T. Hellweg, Curr. Opin. Colloid Interface Sci. 2009, 14, 438.
R. Pelton, J. Colloid Interface Sci. 2010, 348, 673.
Y. Gao, S. C. F. Au-Yeung, C. Wu, Macromolecules 1999, 32, 3674.
M. F. Pittenger, D. E. Discher, B. M. Péault, D. G. Phinney, J. M. Hare, A. I. Caplan, npj Regener. Med. 2019, 4, 22.
C. A. V. Rodrigues, T. G. Fernandes, M. M. Diogo, C. L. da Silva, J. M. S. Cabral, Biotechnol. Adv. 2011, 29, 815.
A. Kumar, B. Starly, Biofabrication 2015, 7, 044103.
R. D. Abbott, D. L. Kaplan, Trends Biotechnol. 2015, 33, 401.
N. Liu, R. Zang, S.-T. Yang, Y. Li, Eng. Life Sci. 2014, 14, 4.
T. L. Place, F. E. Domann, A. J. Case, Free Radical Biol. Med. 2017, 113, 311.
N. V. Chevtchik, M. Fedecostante, J. Jansen, M. Mihajlovic, M. Wilmer, M. Rüth, R. Masereeuw, D. Stamatialis, Eur. J. Pharmacol. 2016, 790, 28.
D. Janke, J. Jankowski, M. Rüth, I. Buschmann, H. D. Lemke, D. Jacobi, P. Knaus, E. Spindler, W. Zidek, K. Lehmann, V. Jankowski, PLoS One 2013, 8, e57227.
D. F. Stamatialis, B. J. Papenburg, M. Gironés, S. Saiful, S. N. M. Bettahalli, S. Schmitmeier, M. Wessling, J. Membr. Sci. 2008, 308, 1.
N. M. S. Bettahalli, H. Steg, M. Wessling, D. Stamatialis, J. Membr. Sci. 2011, 371, 117.
N. M. S. Bettahalli, N. Groen, H. Steg, H. Unadkat, J. d. Boer, C. A. v. Blitterswijk, M. Wessling, D. Stamatialis, J. Tissue Eng. Regener. Med. 2014, 8, 106.
N. M. S. Bettahalli, J. Vicente, L. Moroni, G. A. Higuera, C. A. Van Blitterswijk, M. Wessling, D. F. Stamatialis, Acta Biomater. 2011, 7, 3312.
H. Eghbali, M. M. Nava, D. Mohebbi-Kalhori, M. T. Raimondi, Int. J. Artif. Organs 2016, 39, 1.
R. Pelton, Adv. Colloid Interface Sci. 2000, 85, 1.
D. I. Phua, K. Herman, A. Balaceanu, J. Zakrevski, A. Pich, Langmuir 2016, 32, 3867.
a) M. Kather, M. Skischus, P. Kandt, A. Pich, G. Conrads, S. Neuss, Angew. Chem., Int. Ed. 2017, 56, 2497;
b) M. Kather, M. Skischus, P. Kandt, A. Pich, G. Conrads, S. Neuss, Angew. Chem. 2017, 129, 2537.
J. B. Thorne, G. J. Vine, M. J. Snowden, Colloid Polym. Sci. 2011, 289, 625.
M. Stieger, W. Richtering, J. S. Pedersen, P. Lindner, J. Chem. Phys. 2004, 120, 6197.
M. Zhuang, T. Liu, K. Song, D. Ge, X. Li, Mater. Sci. Eng., C 2015, 55, 410.
J. Ramos, A. Imaz, J. Forcada, Polym. Chem. 2012, 3, 852.
H. Vihola, A. Laukkanen, L. Valtola, H. Tenhu, J. Hirvonen, Biomaterials 2005, 26, 3055.
a) D. Menne, F. Pitsch, J. E. Wong, A. Pich, M. Wessling, Angew. Chem., Int. Ed. 2014, 53, 5706;
b) D. Menne, F. Pitsch, J. E. Wong, A. Pich, M. Wessling, Angew. Chem. 2014, 126, 5814.
M. Barth, M. Wiese, W. Ogieglo, D. Go, A. J. C. Kuehne, M. Wessling, J. Membr. Sci. 2018, 555, 473.
T. Lohaus, P. de Wit, M. Kather, D. Menne, N. E. Benes, A. Pich, M. Wessling, J. Membr. Sci. 2017, 539, 451.
A. S. Curtis, J. V. Forrester, C. McInnes, F. Lawrie, J. Cell Biol. 1983, 97, 1500.
H. Kawaguchi, K. Kisara, T. Takahashi, K. Achiha, M. Yasui, K. Fujimoto, Macromol. Symp. 2000, 151, 591.
Z. Tang, Y. Akiyama, K. Itoga, J. Kobayashi, M. Yamato, T. Okano, Biomaterials 2012, 33, 7405.
E. Décavé, D. Garrivier, Y. Bréchet, B. Fourcade, F. Bruckert, Biophys. J. 2002, 82, 2383.
D. Garrivier, E. Décavé, Y. Bréchet, F. Bruckert, B. Fourcade, Eur. Phys. J. E 2002, 8, 79.
Y. Arisaka, J. Kobayashi, M. Yamato, Y. Akiyama, T. Okano, Biomaterials 2013, 34, 4214.