Scoparone alleviates hepatic fibrosis by inhibiting the TLR-4/NF-κB pathway.
Toll‐like receptor‐4/nuclear factor kappa‐B
hepatic fibrosis
phosphorylation of nuclear factor‐κB
scoparone
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
08 Oct 2020
08 Oct 2020
Historique:
received:
09
01
2020
revised:
07
09
2020
accepted:
09
09
2020
entrez:
22
10
2020
pubmed:
23
10
2020
medline:
23
10
2020
Statut:
aheadofprint
Résumé
The aim of this study was to investigate the role of scoparone (SCO) in hepatic fibrosis. For this, we conducted in vivo and in vitro experiments. In vivo rats that were divided into six groups, control, carbon tetrachloride, and colchicine, as well as SCO groups, SCO50, SCO100, and SCO200 treated with 50, 100, and 200 mg/kg SCO doses, respectively. Furthermore, SCO was shown to inhibit Toll-like receptor-4 (TLR-4)/nuclear factor kappa-B (NF-κB; TLR-4/NF-κB) signals by inhibiting TLR-4, which in turn downregulates the expression of MyD88, promotes NF-κB inhibitor-α, NF-κB inhibitor-β, and NF-κB inhibitor-ε activation, while inhibiting NF-κB inhibitor-ζ. Subsequently, the decrease of phosphorylation of nuclear factor-κB levels leads to the downregulation of the downstream inflammatory factors' tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and IL-1 beta, thus weakening hepatic fibrosis. Notably, the SCO200 treated group presented the most significant improvement. Hence, we conclude that SCO alleviates hepatic fibrosis by inhibiting TLR-4/NF-κB signals.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Science Foundation of Guangxi Province of China
ID : 2017GXNSFAA198218
Organisme : National Science Foundation of Guangxi Province of China
ID : 2017GXNSFAA198326
Organisme : National Science Foundation of Guangxi Province of China
ID : 2018GXNSFAA281040
Organisme : Special funding for 2017 Guangxi BaGui Scholars
Organisme : National Natural Science Foundation of China
ID : 81660104
Organisme : National Natural Science Foundation of China
ID : 81760114
Organisme : National Natural Science Foundation of China
ID : 81860673
Organisme : National Natural Science Foundation of China
ID : 81960779
Organisme : National Natural Science Foundation of China
ID : 62061012
Informations de copyright
© 2020 Wiley Periodicals LLC.
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