Purification of Fab and Fc using papain immobilized cryogel bioreactor separator system.
Bioreactors
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Cryogels
/ chemistry
Electrophoresis, Polyacrylamide Gel
Enzymes, Immobilized
/ chemistry
Humans
Hydrophobic and Hydrophilic Interactions
Immunoglobulin Fab Fragments
/ analysis
Immunoglobulin Fc Fragments
/ analysis
Papain
/ chemistry
Cryogel bioreactor
Fab fragment
Hydrophobic interaction
IgG
Immobilization
Papain
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554
Informations de publication
Date de publication:
20 Nov 2020
20 Nov 2020
Historique:
received:
30
07
2020
revised:
04
09
2020
accepted:
18
09
2020
pubmed:
23
10
2020
medline:
27
4
2021
entrez:
22
10
2020
Statut:
ppublish
Résumé
The continuous bed bioreactor systems have been used for the production of protein therapeutics, such as IgG, using immobilized enzyme in biopharmaceutical applications. We developed macroporous poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) cryogel-based bioreactor matrix using sodium dodecyl sulfate as surfactans in the presence of ethylene glycol dimethacrylate as cross linking agent by bulk polymerization. The developed polyGMA immobilized bioreactor with papain enzyme was used for specific fragmentation of immunoglobulin G. The catalysis efficiency for immobilized enzyme were investigated in comparison with free enzyme. The immobilized papain displayed broad catalytic activity over a variety of conditions, with maximal activity around pH 7.0 and 70 °C. The Michaelis-Menten kinetic constant (K
Identifiants
pubmed: 33091678
pii: S1570-0232(20)31272-1
doi: 10.1016/j.jchromb.2020.122396
pii:
doi:
Substances chimiques
Cryogels
0
Enzymes, Immobilized
0
Immunoglobulin Fab Fragments
0
Immunoglobulin Fc Fragments
0
Papain
EC 3.4.22.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122396Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.