A new ELISA and western blot technique based on recombinant TES antigen and/or larval antigen for the detection of toxocariasis in humans.

Animals Antigens, Helminth / analysis Blotting, Western / methods Enzyme-Linked Immunosorbent Assay / methods Larva / growth & development Toxocara / growth & development Toxocariasis / diagnosis
Antibody detection ELISA Toxocara epidemiology immunoblot serology toxocariasis toxocarosis western blot

Journal

Parasitology
ISSN: 1469-8161
Titre abrégé: Parasitology
Pays: England
ID NLM: 0401121

Informations de publication

Date de publication:
03 2021
Historique:
pubmed: 24 10 2020
medline: 15 10 2021
entrez: 23 10 2020
Statut: ppublish

Résumé

Serological antibody detection by enzyme-linked immunosorbent assay (ELISA)- and immunoblot-based methods constitutes the best indicator of human Toxocara infection. Nevertheless, the availability of serological tests, particularly western blots (WB), evaluated for sensitivity and specificity is limited. Therefore, an Anti-Toxocara-ELISA immunoglobulin g (IgG) prototype (Proto-ELISA) and an Anti-Toxocara-Westernblot (IgG) prototype (Proto-WB) were evaluated by testing 541 human sera pre-determined for Toxocara infection by an established in-house Anti-Toxocara-ELISA (IH-ELISA). To evaluate sensitivity and specificity of the newly developed ELISA and WB prototypes, results were compared to IH-ELISA and a commercial WB (Com-WB). Compared to the IH-ELISA, a sensitivity of 93.1% (229/246) and a specificity of 94.6% (279/295) of the Proto-ELISA with a Cohen's κ of 0.88 were obtained. The sensitivity of the Proto-WB was 76.7% (240/313) and specificity was 99.6% (227/228) with a Cohen's κ of 0.73 compared to those of Com-WB. A comparison to the IH-ELISA revealed 91.5% (225/246) sensitivity and 94.6% (279/295) specificity of the Proto-WB with a Cohen's κ of 0.86. Cross-reactivity was observed for some samples positive for Ascaris and Trichinella spp. in the Proto-ELISA, Proto-WB and Com-WB. Overall, the evaluated ELISA and WB prototypes showed high sensitivity and specificity, indicating high reliability of these newly developed tests.

Identifiants

DOI: 10.1017/S0031182020002085 PMID: 33092663 PMC: PMC7890350
pubmed: 33092663
doi: 10.1017/S0031182020002085
pii: S0031182020002085
pmc: PMC7890350
doi:

Substances chimiques

Antigens, Helminth 0

Types de publication

Evaluation Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

333-340

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Auteurs

Marie-Kristin Raulf (MK)

Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Buenteweg 17, 30559Hannover, Germany.
Institute for Immunology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559Hannover, Germany.
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Buenteweg 17, 30559Hannover, Germany.

Daniela Jordan (D)

Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Buenteweg 17, 30559Hannover, Germany.

Herbert Auer (H)

Department of Medical Parasitology, Center of Pathophysiology, Infectiology and Immunology, Institute of Specific Prophylaxis and Tropical Medicine, Medical University Vienna, Kinderspitalgasse 15, 1090Vienna, Austria.

Jens M Warnecke (JM)

Institute for Experimental Immunology, EUROIMMUN, Seekamp 31, 23560Lübeck, Germany.

Bernd Lepenies (B)

Institute for Immunology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559Hannover, Germany.
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Buenteweg 17, 30559Hannover, Germany.

Christina Strube (C)

Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Buenteweg 17, 30559Hannover, Germany.
ORCID: 0000-0002-9093-1006

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A new ELISA and western blot technique based...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes d'helminthe › A new ELISA and western blot technique based...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › A new ELISA and western blot technique based...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › A new ELISA and western blot technique based...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Larve › A new ELISA and western blot technique based...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Helminthes › Nematoda › Chromadorea › Ascaridida › Ascaridoidea › Toxocara › A new ELISA and western blot technique based...
questionsmedicales.fr › Maladies de l'animal › Parasitoses animales › Helminthoses animales › Toxocarose › A new ELISA and western blot technique based...