The influence of substrate stiffness on osteogenesis of vascular smooth muscle cells.
Osteogenesis
Smooth muscle cells
Substrate stiffness
Vascular calcification
Journal
Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
19
06
2020
revised:
29
08
2020
accepted:
26
09
2020
pubmed:
24
10
2020
medline:
22
6
2021
entrez:
23
10
2020
Statut:
ppublish
Résumé
Vascular stiffening occurs with advanced age and under pathological conditions such as vascular calcification, during which the osteogenesis of smooth muscle cells (SMCs) plays a key role. However, whether the stiffness of cellular microenvironment influences osteogenic responses in vascular SMCs is not well understood. Here, we cultured SMCs on the poly(dimethylsiloxane) (PDMS) substrates with varying stiffness from 0.363 to 2.327 MPa. The cell osteogenic transdifferentiation was induced by β-glycerophosphate. Our findings demonstrated that the extent of osteogenesis in SMCs varied with the substrate stiffness. On three substrate stiffness, cells on the intermediate one (0.909 MPa) showed the highest extent of the osteogenesis based on the expression of osteogenic markers and calcium deposition. Transforming growth factor-β1 and autophagy were involved in this stiffness-dependent process. This work highlights the importance of substrate stiffness to the osteogenesis of vascular SMCs, giving new scientific information for understanding of SMCs-mediated vascular calcification and designing of vascular implants.
Identifiants
pubmed: 33096417
pii: S0927-7765(20)30744-X
doi: 10.1016/j.colsurfb.2020.111388
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111388Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.