Generation of the induced pluripotent stem cell line UHOMi002-A from peripheral blood mononuclear cells of a healthy male donor.


Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
12 2020
Historique:
received: 18 09 2020
revised: 30 09 2020
accepted: 05 10 2020
pubmed: 26 10 2020
medline: 22 6 2021
entrez: 25 10 2020
Statut: ppublish

Résumé

Human pluripotent stem cells (hiPSC) are highly valuable tools to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized 40-year-old healthy male nonsmoking donor. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai Virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers. The reported UHOMi002-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing.

Identifiants

pubmed: 33099111
pii: S1873-5061(20)30338-X
doi: 10.1016/j.scr.2020.102037
pii:
doi:

Substances chimiques

Transcription Factors 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102037

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Mathieu Fieldes (M)

IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France.

Engi Ahmed (E)

IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France; Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France.

Chloé Bourguignon (C)

IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France.

Joffrey Mianné (J)

IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France.

Mélanie Martin (M)

Laboratory of Molecular Genetics, Univ Montpellier, CHU Nîmes, Nîmes, France.

Cécile Arnould (C)

Laboratory of Molecular Genetics, Univ Montpellier, CHU Nîmes, Nîmes, France.

Isabelle Vachier (I)

Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France.

Said Assou (S)

IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France.

John De Vos (J)

IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France; Department of Cell and Tissue Engineering, Saint-Eloi Hospital, Montpellier 34000, France. Electronic address: john.devos@inserm.fr.

Arnaud Bourdin (A)

Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France; PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214. Electronic address: a-bourdin@chu-montpellier.fr.

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Classifications MeSH