Coumarin-based fluorescent biosensor with large linear range for ratiometric measurement of intracellular pH.
Coumarin
Fluorescent probes
Ratiometric imaging
Triazines
pH sensing
Journal
Bioorganic chemistry
ISSN: 1090-2120
Titre abrégé: Bioorg Chem
Pays: United States
ID NLM: 1303703
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
16
07
2020
revised:
11
09
2020
accepted:
08
10
2020
pubmed:
26
10
2020
medline:
17
6
2021
entrez:
25
10
2020
Statut:
ppublish
Résumé
Intracellular pH is a critical parameter involved in cell machinery, and its dysregulation can either cause or signal pathologic states. Currently described fluorescent pH probes are based on single acid-base equilibria, and for this reason are intrinsically unable to capture the wide range of cell pH, usually spanning over more than four units. Here we describe a fluorescent pH biosensor based on a conjugated coumarin-triazine scaffold that is excitable in the visible range, shows pseudo-linear ratiometric response over more than 6 pH units with a single fluorogenic unit, and allows imaging of the whole endo-lysosomal pH landscape of living cells with a single acquisition. The probe can discriminate, on the basis of intracellular acidity, between physiologic and tumor cells, being potentially suitable in perspective as diagnostic tool.
Identifiants
pubmed: 33099166
pii: S0045-2068(20)31670-9
doi: 10.1016/j.bioorg.2020.104372
pii:
doi:
Substances chimiques
Coumarins
0
Fluorescent Dyes
0
Triazines
0
coumarin
A4VZ22K1WT
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104372Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.