Comparative Study for Measurement of Residual Leucocytes in Leucodepleted Red Blood Cells by Two Different Methods.
Filtration
Flowcytometry
Leukodepletion
Leukoreduction
Nageotte
True count beads
Journal
Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion
ISSN: 0971-4502
Titre abrégé: Indian J Hematol Blood Transfus
Pays: India
ID NLM: 9425818
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
17
04
2020
accepted:
20
07
2020
entrez:
26
10
2020
pubmed:
27
10
2020
medline:
27
10
2020
Statut:
ppublish
Résumé
Estimation of residual leukocytes in blood components after leukodepletion is crucial for assessment of quality. Flow cytometry (FC) and Nageotte hemocytometer are the most widely accepted methods for counting residual white blood cells (rWBCs) in leucocyte-depleted (LD) blood components. The objective of this study was to compare use of Nageotte counting chamber and FC for quality control of leukodepleted red cell units. A prospective, observational study was conducted in the department of Transfusion Medicine. A total of 80 whole blood donations from healthy donors were subjected to testing by FC and Nageotte hemocytometer for estimation of rWBC in duplicate. Additionally, ten personnel attempted a survey for ease of use of FC. Number of rWBC detected by flow cytometer were between 1 WBC/μL and 28 WBCs/μL whereas that detected by Nageotte's chamber were between 0 WBC/μL (lowest) and 5 WBCs/μL. Coefficient of variation was found to be 87.36% by Nageotte hemocytometer method and 43.26% by FC. Linear regression analysis did not show any correlation (R-squared = 0.01,
Identifiants
pubmed: 33100719
doi: 10.1007/s12288-020-01325-5
pii: 1325
pmc: PMC7572992
doi:
Types de publication
Journal Article
Langues
eng
Pagination
740-744Informations de copyright
© Indian Society of Hematology and Blood Transfusion 2020.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare no conflict of interest.
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