The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism.
amyloid fiber
antimicrobial peptide
gradual release
peptide-lipid interaction
supramolecular assembly
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2020
2020
Historique:
received:
13
01
2020
accepted:
18
08
2020
entrez:
26
10
2020
pubmed:
27
10
2020
medline:
22
6
2021
Statut:
epublish
Résumé
Magainin 2 and PGLa are antimicrobial peptides found together in frog skin secretions. When added as a mixture they show an order of magnitude increase in antibacterial activity and in model membrane permeation assays. Here we demonstrate that both peptides can form fibers with beta-sheet/turn signature in ATR-FTIR- and CD-spectroscopic analyses, but with different morphologies in EM images. Whereas, fiber formation results in acute reduction of the antimicrobial activity of the individual peptides, the synergistic enhancement of activity remains for the equimolar mixture of PGLa and magainin 2 also after fibril formation. The biological significance and potential applications of such supramolecular aggregates are discussed.
Identifiants
pubmed: 33102247
doi: 10.3389/fcimb.2020.526459
pmc: PMC7554302
doi:
Substances chimiques
Anti-Bacterial Agents
0
Anti-Infective Agents
0
Magainins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
526459Informations de copyright
Copyright © 2020 Juhl, Glattard, Lointier, Bampilis and Bechinger.
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