Comparison of AlphaLISA and RIA assays for measurement of wool cortisol concentrations.
AlphaLISA
Animal physiology
Animal science
Biological sciences
Endocrinology
Glucocorticoids
Lamb
RIA
Sheep
Veterinary medicine
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
22
04
2020
revised:
31
07
2020
accepted:
08
10
2020
entrez:
26
10
2020
pubmed:
27
10
2020
medline:
27
10
2020
Statut:
epublish
Résumé
Radioimmunoassay (RIA) methods have always represented a technique of choice for the determination of steroids in biological samples. The Amplified Luminescent Proximity Homogenous Assay-Linked Immunosorbent Assay (AlphaLISA) is now emerging as the new-generation immunoassay technology that does not require washing/separation steps. The aim of this study was to adapt the Perkin-Elmer's AlphaLISA kit for wool cortisol and compare it with a RIA wool cortisol assay. Wool from lambs, 35 at birth (A0) and 54 at two months old (A2), was collected and each extract was evaluated for wool cortisol concentrations (HCC) both by RIA and AlphaLISA immunoassay. The two methods showed good precision, sensitivity and specificity for determining HCC. Both methods were able to detect significant differences between the high and the low HCC assessed in lambs at A0 and A2 (
Identifiants
pubmed: 33102853
doi: 10.1016/j.heliyon.2020.e05230
pii: S2405-8440(20)32073-9
pmc: PMC7569336
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e05230Informations de copyright
© 2020 The Authors.
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