The relationship of membrane stiffness, cytoskeleton structure and storage time of pRBCs.
cytoskeleton nanostructure
long-term storage
membrane stiffness
pRBCs
storage lesion
Journal
Vox sanguinis
ISSN: 1423-0410
Titre abrégé: Vox Sang
Pays: England
ID NLM: 0413606
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
revised:
10
09
2020
received:
06
08
2020
accepted:
22
09
2020
pubmed:
27
10
2020
medline:
29
6
2021
entrez:
26
10
2020
Statut:
ppublish
Résumé
In clinical practice, it has been shown that transfusion of packed red blood cells (pRBCs) with late shelf life increases the risk of post-transfusion complications. To study relationship of membrane stiffness, cytoskeleton structure and storage time of pRBCs. pRBCs were processed and stored according to blood bank procedure, for 42 days, at +4°C; pRBC samples were taken on days 3, 12, 19, 21, 24, 28, 35 and 42. Cytoskeleton images and membrane stiffness were studied using atomic force microscope. In the course of the pRBC storage, the cytoskeleton network configuration underwent structural changes. Simultaneously, pRBC membrane stiffness was increasing, with the correlation coefficient 0·88. Until 19 days, the stiffness grew slowly, in 19-24 days there occurred a transition period, after which its growth rate was three times higher than the initial. A chain of pathological processes developed in pRBC during long storage: pH reduction (linked to increased oxidative stress), then cytoskeletal destruction and an associated increase in pRBC membrane stiffness. During prolonged storage of pRBCs and their acidification, there is a progression of pRBC cytoskeletal changes and associated increase of membrane stiffness, observed to increase in rate after days 19-24. Mutual measurements of cytoskeletal integrity and membrane stiffness may be useful quality assessment tool to study the molecular mechanisms of RBC structural degradation during storage.
Sections du résumé
BACKGROUND AND OBJECTIVES
OBJECTIVE
In clinical practice, it has been shown that transfusion of packed red blood cells (pRBCs) with late shelf life increases the risk of post-transfusion complications.
OBJECTIVE
OBJECTIVE
To study relationship of membrane stiffness, cytoskeleton structure and storage time of pRBCs.
MATERIALS AND METHODS
METHODS
pRBCs were processed and stored according to blood bank procedure, for 42 days, at +4°C; pRBC samples were taken on days 3, 12, 19, 21, 24, 28, 35 and 42. Cytoskeleton images and membrane stiffness were studied using atomic force microscope.
RESULTS
RESULTS
In the course of the pRBC storage, the cytoskeleton network configuration underwent structural changes. Simultaneously, pRBC membrane stiffness was increasing, with the correlation coefficient 0·88. Until 19 days, the stiffness grew slowly, in 19-24 days there occurred a transition period, after which its growth rate was three times higher than the initial. A chain of pathological processes developed in pRBC during long storage: pH reduction (linked to increased oxidative stress), then cytoskeletal destruction and an associated increase in pRBC membrane stiffness.
CONCLUSION
CONCLUSIONS
During prolonged storage of pRBCs and their acidification, there is a progression of pRBC cytoskeletal changes and associated increase of membrane stiffness, observed to increase in rate after days 19-24. Mutual measurements of cytoskeletal integrity and membrane stiffness may be useful quality assessment tool to study the molecular mechanisms of RBC structural degradation during storage.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
405-415Subventions
Organisme : Ministry of Science and Higher Education of the Russian Federation, Project 5-100
Informations de copyright
© 2020 International Society of Blood Transfusion.
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