Neutrophils Enable Local and Non-Invasive Liposome Delivery to Inflamed Skeletal Muscle and Ischemic Heart.
inflammation
liposomes
methotrexate
neutrophils
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
26
05
2020
revised:
03
09
2020
pubmed:
27
10
2020
medline:
12
8
2021
entrez:
26
10
2020
Statut:
ppublish
Résumé
Uncontrolled inflammation is a major pathological factor underlying a range of diseases including autoimmune conditions, cardiovascular disease, and cancer. Improving localized delivery of immunosuppressive drugs to inflamed tissue in a non-invasive manner offers significant promise to reduce severe side effects caused by systemic administration. Here, a neutrophil-mediated delivery system able to transport drug-loaded nanocarriers to inflamed tissue by exploiting the inherent ability of neutrophils to migrate to inflammatory tissue is reported. This hybrid system (neutrophils loaded with liposomes ex vivo) efficiently migrates in vitro following an inflammatory chemokine gradient. Furthermore, the triggered release of loaded liposomes and reuptake by target macrophages is studied. The migratory behavior of liposome-loaded neutrophils is confirmed in vivo by demonstrating the delivery of drug-loaded liposomes to an inflamed skeletal muscle in mice. A single low-dose injection of the hybrid system locally reduces inflammatory cytokine levels. Biodistribution of liposome-loaded neutrophils in a human-disease-relevant myocardial ischemia reperfusion injury mouse model after i.v. injection confirms the ability of injected neutrophils to carry loaded liposomes to inflammation sites. This strategy shows the potential of nanocarrier-loaded neutrophils as a universal platform to deliver anti-inflammatory drugs to promote tissue regeneration in inflammatory diseases.
Identifiants
pubmed: 33103807
doi: 10.1002/adma.202003598
pmc: PMC7613371
mid: EMS124739
doi:
Substances chimiques
Liposomes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2003598Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N503952/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R015651/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 209121/Z/17/Z
Pays : United Kingdom
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : Marie Skłodowska Curie Individual Fellowship
ID : 746980
Organisme : BHF
ID : PG/16/93/32345
Organisme : Wellcome Trust
ID : 209121
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RM/17/1/33377
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 209121_Z_17_Z
Pays : United Kingdom
Organisme : British Heart Foundation
ID : PG/16/93/32345
Pays : United Kingdom
Organisme : European Union's Horizon 2020 research and innovation programme
ID : 681137
Organisme : European Commission H2020
ID : 794059
Organisme : China Scholarship Council
Organisme : BHF
ID : CH/08/002/29257
Organisme : BHF
ID : RM/17/1/33377
Informations de copyright
© 2020 The Authors. Published by Wiley-VCH GmbH.
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