Performance of Xpert HPV on Self-collected Vaginal Samples for Cervical Cancer Screening Among Women in South Africa.


Journal

Journal of lower genital tract disease
ISSN: 1526-0976
Titre abrégé: J Low Genit Tract Dis
Pays: United States
ID NLM: 9704963

Informations de publication

Date de publication:
01 Jan 2021
Historique:
pubmed: 27 10 2020
medline: 9 9 2021
entrez: 26 10 2020
Statut: ppublish

Résumé

Self-sampling may increase access to cervical cancer screening in low-resource settings. Using Xpert HPV, we compared test performance of self- and clinician-collected samples in HIV-positive and HIV-negative women in South Africa. Three hundred thirty HIV-positive and 375 HIV-negative women in the screening group and 202 HIV-negative and 200 HIV-positive women in the referral group, aged 30-65 years, participated in the study. All women self-collected a vaginal sample, and then, a cervical sample was collected by a clinician (both tested using Xpert HPV), followed by colposcopic examination and collection of histologic specimens. There was good agreement between self- and clinician-collected samples for detection of any high-risk human papillomavirus (HPV, κ = 0.72 [95% CI = 0.669-0.771]). Prevalence of HPV and sensitivity of the test to detect cervical intraepithelial neoplasia 2+ was similar in self- and clinician-collected samples. Specificity was lower in self-collected than in clinician-collected samples in both HIV-negative (self: 77.5% [95% CI = 72.8-81.8] vs clinician: 86.9% [95% CI = 82.9-90.2]) and HIV-positive (self: 44.0% [95% CI = 38.0-50.1] vs clinician: 59.7% [95% CI = 53.6-65.6]) women. Restricting the definition of screen-positive to 3 of 5 channels on HPV Xpert improved specificity in both HIV-negative (self: 83.2% [95% CI = 78.8-87.0] vs clinician: 89.7% [95% CI = 86.1-92.7]) and HIV-positive (self: 54.2% [95% CI = 48.1-60.2] vs clinician: 67.4% [95% CI = 61.5-72.9]) women. The self-collected sample had good agreement with the clinician-collected sample for the detection of HPV, and restricting the HPV types may improve the specificity in HIV-positive women.

Identifiants

pubmed: 33105451
pii: 00128360-202101000-00003
doi: 10.1097/LGT.0000000000000575
pmc: PMC7748046
mid: NIHMS1632427
doi:

Types de publication

Comparative Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

15-21

Subventions

Organisme : NCI NIH HHS
ID : UH2 CA189908
Pays : United States
Organisme : NCI NIH HHS
ID : UH3 CA189908
Pays : United States

Informations de copyright

Copyright © 2020, ASCCP.

Déclaration de conflit d'intérêts

CSB, DP, and SC declare that they receive salary, benefits, and equity from Danaher Corp. Cepheid is a wholly owned entity of Danaher Corporation. TCW reports that he serves as a consultant in clinical trial design and as an expert pathologist for human papillomavirus vaccine or diagnostic trials for Becton Dickinson, BD Life Sciences-Diagnostic Systems, Roche, and Inovio Pharmaceuticals and as a speaker for Roche and Becton Dickinson, and BD Life Sciences-Diagnostic Systems. The other authors have declared they have no conflicts of interest.

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Auteurs

Rosalind Boa (R)

Department of Obstetrics and Gynaecology, Old Main Building, Groote Schuur Hospital, Observatory, University of Cape Town, Cape Town, South Africa.

Cecilia Svanholm-Barrie (C)

Cepheid, Inc, Solna, Sweden.

David Persing (D)

Cepheid, Inc, Sunnyvale, CA.

Scott Campbell (S)

Cepheid, Inc, Sunnyvale, CA.

Wei-Yann Tsai (WY)

Department of Biostatistics, Mailman School of Public Health, Columbia University Irving Medical Centre, New York, NY.

Thomas C Wright (TC)

Department of Pathology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Centre, New York, NY.

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