Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results.

Comet Assay / methods Consensus Guideline Adherence / statistics & numerical data Humans Laboratories Research Design

Journal

Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307

Informations de publication

Date de publication:
12 2020
Historique:
received: 11 02 2020
accepted: 18 08 2020
pubmed: 28 10 2020
medline: 2 2 2021
entrez: 27 10 2020
Statut: ppublish

Résumé

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.

Identifiants

DOI: 10.1038/s41596-020-0398-1 PMID: 33106678 PMC: PMC7688437
pubmed: 33106678
doi: 10.1038/s41596-020-0398-1
pii: 10.1038/s41596-020-0398-1
pmc: PMC7688437
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

3817-3826

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Auteurs

Peter Møller (P)

Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark. pemo@sund.ku.dk.
ORCID: 0000-0002-2021-1249

Amaya Azqueta (A)

Department of Pharmacology and Toxicology, University of Navarra, Pamplona, Spain.
IdiSNA, Navarra Institute for Health Research, Pamplona, Spain.

Elisa Boutet-Robinet (E)

Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, Toulouse, France.
ORCID: 0000-0002-5223-4568

Gudrun Koppen (G)

VITO-HEALTH, Mol, Belgium.

Stefano Bonassi (S)

Unit of Clinical and Molecular Epidemiology IRCCS San Raffaele Pisana, Rome, Italy.
Department of Human Sciences and Quality of Life Promotion, San Raffaele University, Rome, Italy.

Mirta Milić (M)

Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.

Goran Gajski (G)

Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
ORCID: 0000-0002-1886-1453

Solange Costa (S)

Environmental Health Department, National Institute of Health Dr Ricardo Jorge, Porto, Portugal.
Epidemiological Research Unit (EPIUnit), Instituto de Saúde Pública, Universidade do Porto, Porto, Portugal.

João Paulo Teixeira (JP)

Environmental Health Department, National Institute of Health Dr Ricardo Jorge, Porto, Portugal.
Epidemiological Research Unit (EPIUnit), Instituto de Saúde Pública, Universidade do Porto, Porto, Portugal.
ORCID: 0000-0001-8693-5250

Cristiana Costa Pereira (C)

Environmental Health Department, National Institute of Health Dr Ricardo Jorge, Porto, Portugal.
Epidemiological Research Unit (EPIUnit), Instituto de Saúde Pública, Universidade do Porto, Porto, Portugal.
ORCID: 0000-0002-2146-1223

Maria Dusinska (M)

Health Effects Laboratory, Department of Environmental Chemistry, Norwegian Institute for Air Research (NILU), Kjeller, Norway.

Roger Godschalk (R)

Department of Pharmacology & Toxicology, School for Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, Maastricht, the Netherlands.

Gunnar Brunborg (G)

Section of Molecular Toxicology, Department of Environmental Health, Norwegian Institute of Public Health Skøyen, Oslo, Norway.

Kristine B Gutzkow (KB)

Section of Molecular Toxicology, Department of Environmental Health, Norwegian Institute of Public Health Skøyen, Oslo, Norway.

Lisa Giovannelli (L)

Department of Neuroscience, Psychology, Pharmacology and Child Health (NEUROFARBA), Section Pharmacology and Toxicology, University of Florence, Florence, Italy.

Marcus S Cooke (MS)

Oxidative Stress Group, Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL, USA.

Elke Richling (E)

Food Chemistry & Toxicology, Department of Chemistry, University of Kaiserslautern, Kaiserslautern, Germany.

Blanca Laffon (B)

Universidade da Coruña, Grupo DICOMOSA, Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Psicología, Facultad de Ciencias de la Educación, Coruña, Spain.
Instituto de Investigación Biomédica de A Coruña (INIBIC), AE CICA-INIBIC, Coruña, Spain.
ORCID: 0000-0001-7649-2599

Vanessa Valdiglesias (V)

Universidade da Coruña, Grupo DICOMOSA, Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Psicología, Facultad de Ciencias de la Educación, Coruña, Spain.
Instituto de Investigación Biomédica de A Coruña (INIBIC), AE CICA-INIBIC, Coruña, Spain.

Nursen Basaran (N)

Department of Toxicology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.

Cristian Del Bo' (C)

Division of Human Nutrition, Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, Milan, Italy.

Bojana Zegura (B)

Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia.

Matjaz Novak (M)

Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia.

Helga Stopper (H)

Institute of Pharmacology and Toxicology, University of Würzburg, Würzburg, Germany.

Pavel Vodicka (P)

Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
Biomedical Center, Medical Faculty in Pilsen, Charles University in Prague, Prague, Czech Republic.

Sona Vodenkova (S)

Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
Biomedical Center, Medical Faculty in Pilsen, Charles University in Prague, Prague, Czech Republic.

Vanessa Moraes de Andrade (VM)

Laboratory of Translational Biomedicine, University of Southern Santa Catarina, UNESC, Criciúma, Brazil.
ORCID: 0000-0002-8474-4482

Monika Sramkova (M)

Department of Nanobiology, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Bratislava, Slovakia.

Alena Gabelova (A)

Department of Nanobiology, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Bratislava, Slovakia.

Andrew Collins (A)

Department of Nutrition, University of Oslo, Oslo, Norway.

Sabine A S Langie (SAS)

Department of Pharmacology & Toxicology, School for Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, Maastricht, the Netherlands.
Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Tests de toxicité › Tests de mutagénicité › Test des comètes › Minimum Information for Reporting on the Comet...
questionsmedicales.fr › Phénomènes psychologiques › Processus mentaux › Pensée (activité mentale) › Prise de décision › Consensus › Minimum Information for Reporting on the Comet...
questionsmedicales.fr › Qualité, accès, évaluation des soins de santé › Qualité des soins de santé › Mécanismes d'évaluation des soins de santé › Adhésion aux directives › Minimum Information for Reporting on the Comet...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Minimum Information for Reporting on the Comet...
questionsmedicales.fr › Établissements, main d'oeuvre et services de soins de santé › Établissements de santé › Laboratoires › Minimum Information for Reporting on the Comet...
questionsmedicales.fr › Disciplines des sciences naturelles › Science › Recherche › Plan de recherche › Minimum Information for Reporting on the Comet...