Effect of Sex Steroid Hormones on Tongue Cancer Cells
Cell Line, Tumor
Cell Movement
/ drug effects
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Dihydrotestosterone
/ pharmacology
Estradiol
/ pharmacology
Estrogen Receptor beta
/ metabolism
Gonadal Steroid Hormones
/ pharmacology
Humans
Matrix Metalloproteinase 8
/ metabolism
Neoplasm Invasiveness
Tongue Neoplasms
/ drug therapy
MMP8
Oral tongue squamous cell carcinoma
estradiol
sex hormones
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
09
09
2020
revised:
22
09
2020
accepted:
24
09
2020
entrez:
28
10
2020
pubmed:
29
10
2020
medline:
5
11
2020
Statut:
ppublish
Résumé
Tongue cancer is more common in men than in women. Yet the effects of sex steroid hormones on the behaviour of oral tongue squamous cell carcinoma (OTSCC) are not well known. Matrix metalloproteinase 8 (MMP8) is expressed in OTSCC and can degrade estrogen receptors (ERs). Western blot was used to examine the levels of ERβ in OTSCC cell lines (HSC-3 and SCC-25). We evaluated the effects of estradiol and dihydrotestosterone (DHT) on HSC-3 and SCC-25 cell migration, invasion and viability. The effect of estradiol on the invasion of MMP8-overexpressing (MMP8 Both HSC-3 and SCC-25 cells expressed ERβ. In scratch assay, estradiol, but not DHT, reduced the migration and invasion of HSC-3 and SCC-25 cells. MMP8 Estradiol inhibited the migration and invasion of OTSCC cells, whereas DHT had no effect. Our data suggest that MMP8 does not modulate the effect of estradiol in OTSCC cells. However, the sex difference in OTSCC incidence might partly be due to protective actions of estradiol in epithelial cell carcinogenesis.
Sections du résumé
BACKGROUND
BACKGROUND
Tongue cancer is more common in men than in women. Yet the effects of sex steroid hormones on the behaviour of oral tongue squamous cell carcinoma (OTSCC) are not well known. Matrix metalloproteinase 8 (MMP8) is expressed in OTSCC and can degrade estrogen receptors (ERs).
MATERIALS AND METHODS
METHODS
Western blot was used to examine the levels of ERβ in OTSCC cell lines (HSC-3 and SCC-25). We evaluated the effects of estradiol and dihydrotestosterone (DHT) on HSC-3 and SCC-25 cell migration, invasion and viability. The effect of estradiol on the invasion of MMP8-overexpressing (MMP8
RESULTS
RESULTS
Both HSC-3 and SCC-25 cells expressed ERβ. In scratch assay, estradiol, but not DHT, reduced the migration and invasion of HSC-3 and SCC-25 cells. MMP8
CONCLUSION
CONCLUSIONS
Estradiol inhibited the migration and invasion of OTSCC cells, whereas DHT had no effect. Our data suggest that MMP8 does not modulate the effect of estradiol in OTSCC cells. However, the sex difference in OTSCC incidence might partly be due to protective actions of estradiol in epithelial cell carcinogenesis.
Identifiants
pubmed: 33109541
pii: 40/11/6029
doi: 10.21873/anticanres.14624
doi:
Substances chimiques
Estrogen Receptor beta
0
Gonadal Steroid Hormones
0
Dihydrotestosterone
08J2K08A3Y
Estradiol
4TI98Z838E
Matrix Metalloproteinase 8
EC 3.4.24.34
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6029-6037Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.