Geometry and the Organizational Principle of Spine Synapses along a Dendrite.
FIB/SEM
dendritic spine
electron microscopy
glutamate uncaging
postsynaptic density
simulation
Journal
eNeuro
ISSN: 2373-2822
Titre abrégé: eNeuro
Pays: United States
ID NLM: 101647362
Informations de publication
Date de publication:
Historique:
received:
08
06
2020
revised:
02
10
2020
accepted:
07
10
2020
pubmed:
29
10
2020
medline:
22
6
2021
entrez:
28
10
2020
Statut:
epublish
Résumé
Precise information on synapse organization in a dendrite is crucial to understanding the mechanisms underlying voltage integration and the variability in the strength of synaptic inputs across dendrites of different complex morphologies. Here, we used focused ion beam/scanning electron microscope (FIB/SEM) to image the dendritic spines of mice in the hippocampal CA1 region, CA3 region, somatosensory cortex, striatum, and cerebellum (CB). Our results show that the spine geometry and dimensions differ across neuronal cell types. Despite this difference, dendritic spines were organized in an orchestrated manner such that the postsynaptic density (PSD) area per unit length of dendrite scaled positively with the dendritic diameter in CA1 proximal stratum radiatum (PSR), cortex, and CB. The ratio of the PSD area to neck length was kept relatively uniform across dendrites of different diameters in CA1 PSR. Computer simulation suggests that a similar level of synaptic strength across different dendrites in CA1 PSR enables the effective transfer of synaptic inputs from the dendrites toward soma. Excitatory postsynaptic potentials (EPSPs), evoked at single spines by glutamate uncaging and recorded at the soma, show that the neck length is more influential than head width in regulating the EPSP magnitude at the soma. Our study describes thorough morphologic features and the organizational principles of dendritic spines in different brain regions.
Identifiants
pubmed: 33109633
pii: ENEURO.0248-20.2020
doi: 10.1523/ENEURO.0248-20.2020
pmc: PMC7772515
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 Parajuli et al.
Références
Cell. 2015 Oct 8;163(2):456-92
pubmed: 26451489
J Neurosci. 2011 Aug 24;31(34):12129-38
pubmed: 21865455
Neuron. 2016 Feb 17;89(4):784-99
pubmed: 26853305
Nature. 2011 Mar 10;471(7337):177-82
pubmed: 21390124
J Microsc. 2005 Apr;218(Pt 1):52-61
pubmed: 15817063
Nature. 2004 Jun 17;429(6993):761-6
pubmed: 15190253
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
J Neurosci. 1983 Feb;3(2):383-8
pubmed: 6822869
Nat Neurosci. 2000 Sep;3(9):895-903
pubmed: 10966620
J Neurosci. 1997 Aug 1;17(15):5858-67
pubmed: 9221783
Neuron. 2009 Jul 30;63(2):171-7
pubmed: 19640476
Nat Neurosci. 2001 Nov;4(11):1086-92
pubmed: 11687814
Brain Res Bull. 2017 Mar;129:3-11
pubmed: 27491624
Science. 1996 May 3;272(5262):716-9
pubmed: 8614831
Microscopy (Oxf). 2020 Jul 30;69(4):196-213
pubmed: 32244257
Hippocampus. 2011 Apr;21(4):354-73
pubmed: 20101601
Nat Commun. 2018 Jan 29;9(1):422
pubmed: 29379017
Curr Opin Neurobiol. 2003 Jun;13(3):372-83
pubmed: 12850223
J Neurophysiol. 2009 Oct;102(4):2288-302
pubmed: 19675296
Neuron. 1998 Sep;21(3):545-59
pubmed: 9768841
Ann N Y Acad Sci. 1962 Mar 2;96:1071-92
pubmed: 14490041
Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):E1986-E1995
pubmed: 28209776
Neuron. 2017 Nov 15;96(4):871-882.e5
pubmed: 29107520
J Neurosci. 2004 Dec 8;24(49):11046-56
pubmed: 15590921
Methods Mol Biol. 2019;1941:17-27
pubmed: 30707424
Nat Neurosci. 2015 Dec;18(12):1713-21
pubmed: 26605882
J Cell Sci. 1969 Sep;5(2):509-29
pubmed: 5362339
Cell. 2015 Jul 30;162(3):648-61
pubmed: 26232230
J Neurosci. 1989 Aug;9(8):2982-97
pubmed: 2769375
Science. 2012 Jan 20;335(6066):353-6
pubmed: 22267814
Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18799-804
pubmed: 17132736
Neuron. 2002 Jan 31;33(3):325-40
pubmed: 11832222
Science. 2016 Sep 2;353(6303):1037-1040
pubmed: 27516412
J Comp Neurol. 1992 Sep 8;323(2):137-52
pubmed: 1401253
Curr Opin Neurobiol. 2008 Jun;18(3):321-31
pubmed: 18804167
Nat Neurosci. 2018 Mar;21(3):353-363
pubmed: 29459763
Sci Rep. 2012;2:485
pubmed: 22761993
Exp Neurol. 1981 Nov;74(2):621-7
pubmed: 7297640
Science. 2014 Sep 26;345(6204):1616-20
pubmed: 25258080
Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17961-6
pubmed: 17093040
Cell Rep. 2015 Jan 13;10(2):162-9
pubmed: 25558061
Cereb Cortex. 2020 Mar 21;30(2):730-752
pubmed: 31268532
Nature. 2003 Apr 3;422(6931):518-22
pubmed: 12673250
eNeuro. 2020 Aug 27;7(4):
pubmed: 32817196
Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):E2895-904
pubmed: 24982196
Elife. 2015 Nov 30;4:e10778
pubmed: 26618907
J Neurosci. 1992 Jul;12(7):2685-705
pubmed: 1613552
Neuron. 2013 Dec 18;80(6):1451-63
pubmed: 24360547
Nat Rev Neurosci. 2006 Jul;7(7):575-83
pubmed: 16791146
J Neurobiol. 2003 Aug;56(2):95-112
pubmed: 12838576
J Neurosci. 1988 Dec;8(12):4455-69
pubmed: 3199186
Biochem Biophys Res Commun. 2020 Sep 10;530(1):130-135
pubmed: 32828274
Neuron. 2018 Jul 25;99(2):275-282.e3
pubmed: 29983327