A pH-sensitive eosin-block copolymer delivers proteins intracellularly.
Animals
Bacterial Toxins
/ antagonists & inhibitors
Cell Survival
/ drug effects
Eosine Yellowish-(YS)
/ chemistry
Erythrocytes
/ drug effects
Gene Editing
Heat-Shock Proteins
/ antagonists & inhibitors
Hemolysin Proteins
/ antagonists & inhibitors
Hydrogen-Ion Concentration
Mice
Models, Molecular
Molecular Structure
Polyethylene Glycols
/ chemistry
Journal
Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838
Informations de publication
Date de publication:
25 Nov 2020
25 Nov 2020
Historique:
pubmed:
29
10
2020
medline:
20
5
2021
entrez:
28
10
2020
Statut:
ppublish
Résumé
There is great interest in developing strategies to deliver proteins into the cytoplasm of cells. We report here a PEG-poly-eosin block copolymer (PEG-pEosin) that can encapsulate proteins and release them in active form under mildly acidic conditions. A PEG-pEosin formulation composed of Cre and the endosomolytic protein LLO efficiently performed gene editing in cells and in the brains of mice after an intracranial injection.
Substances chimiques
Bacterial Toxins
0
Heat-Shock Proteins
0
Hemolysin Proteins
0
Polyethylene Glycols
3WJQ0SDW1A
hlyA protein, Listeria monocytogenes
R06ZRQ1YX9
Eosine Yellowish-(YS)
TDQ283MPCW
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM