Isoflurane anesthesia disrupts the cortical metabolome.
Anesthesia
Anesthetics, Inhalation
/ administration & dosage
Animals
Chromatography, Liquid
Consciousness
/ drug effects
Isoflurane
/ administration & dosage
Male
Metabolome
/ drug effects
Mice
Mice, Inbred C57BL
Microdialysis
Nerve Net
/ drug effects
Prefrontal Cortex
/ drug effects
Tandem Mass Spectrometry
Wakefulness
/ drug effects
in vivo microdialysis
liquid chromatography-dual mass spectrometry
prefrontal cortex
states of consciousness
untargeted metabolomics
Journal
Journal of neurophysiology
ISSN: 1522-1598
Titre abrégé: J Neurophysiol
Pays: United States
ID NLM: 0375404
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
pubmed:
29
10
2020
medline:
21
10
2021
entrez:
28
10
2020
Statut:
ppublish
Résumé
Identifying similarities and differences in the brain metabolome during different states of consciousness has broad relevance for neuroscience and state-dependent autonomic function. This study focused on the prefrontal cortex (PFC) as a brain region known to modulate states of consciousness. Anesthesia was used as a tool to eliminate wakefulness. Untargeted metabolomic analyses were performed on microdialysis samples obtained from mouse PFC during wakefulness and during isoflurane anesthesia. Analyses detected 2,153 molecules, 91 of which could be identified. Analytes were grouped as detected during both wakefulness and anesthesia (
Identifiants
pubmed: 33112692
doi: 10.1152/jn.00375.2020
pmc: PMC7814899
doi:
Substances chimiques
Anesthetics, Inhalation
0
Isoflurane
CYS9AKD70P
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2012-2021Subventions
Organisme : Office of Extramural Research, National Institutes of Health (OER)
ID : HL65272
Pays : International
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