NLRP3 Inflammasome Activation in Hemodialysis and Hypertensive Patients with Intact Kidney Function.


Journal

Toxins
ISSN: 2072-6651
Titre abrégé: Toxins (Basel)
Pays: Switzerland
ID NLM: 101530765

Informations de publication

Date de publication:
26 10 2020
Historique:
received: 11 09 2020
revised: 16 10 2020
accepted: 22 10 2020
entrez: 29 10 2020
pubmed: 30 10 2020
medline: 9 7 2021
Statut: epublish

Résumé

Hypertension is not only an integrative characteristic of hemodialysis (HD) patients but is also very common in the general population. There is evidence that the inflammatory cytokine IL-β, regulated by the NLRP3 inflammasome via caspase-1, contributes to the hypertensive setting. Therefore, we investigated in an observational pilot study whether IL-1β secretion and inflammatory cell death (pyroptosis) are different in HD and hypertensive patients with intact kidney function. Twenty HD patients were age-, gender-, and diabetes-mellitus-matched to patients with hypertension and intact kidney function. Caspase-1 activity and pyroptosis rates were measured by flow cytometry. IL-1β was determined by qPCR and the ELISA technique. The inflammatory status (CRP) did not differ between both groups; however, the body mass index, a classical cardiovascular risk factor, was significantly elevated in blood pressure (BP) patients. BP patients had a higher frequency of caspase-1-positive monocytes compared to HD (

Identifiants

pubmed: 33114648
pii: toxins12110675
doi: 10.3390/toxins12110675
pmc: PMC7693185
pii:
doi:

Substances chimiques

Inflammasomes 0
Interleukin-1beta 0
NLR Family, Pyrin Domain-Containing 3 Protein 0
NLRP3 protein, human 0

Types de publication

Journal Article Observational Study

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Christof Ulrich (C)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Susann Wildgrube (S)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Roman Fiedler (R)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Eric Seibert (E)

Nephrologische Kooperation Villingen-Schwenningen, 78052 Villingen-Schwenningen, Germany.

Leonie Kneser (L)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Sylvia Fick (S)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Christoph Schäfer (C)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Silke Markau (S)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Bogusz Trojanowicz (B)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Matthias Girndt (M)

Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

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Classifications MeSH