Prognostic Stratification of Bladder Cancer Patients with a MicroRNA-based Approach.

bladder cancer liquid biopsy microRNAs prognostic stratification

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
26 Oct 2020
Historique:
received: 01 10 2020
revised: 20 10 2020
accepted: 21 10 2020
entrez: 29 10 2020
pubmed: 30 10 2020
medline: 30 10 2020
Statut: epublish

Résumé

Robust non-invasive tests for prognostic stratification of bladder cancer (BCa) patients are in high demand. Following a comprehensive analysis of studies on BCa, we selected a panel of 29 microRNAs (miRNAs) and analyzed their levels in urine and plasma samples in a prospective cohort of 63 BCa patients (32 at high risk of recurrence and 31 low-risk cases) and 37 healthy controls using RT-qPCR. To design an assay suitable for large-scale testing, we applied a hierarchical pipeline to select the miRNAs that were not affected by confounding factors such as haematuria and urine specific gravity, and exceeded stringent cut-off criteria (fold change >2.5 and

Identifiants

pubmed: 33114775
pii: cancers12113133
doi: 10.3390/cancers12113133
pmc: PMC7692037
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Istituto Oncologico Veneto
ID : 5x1000
Organisme : University of Padova
ID : SID16_01 and BIRD 205490

Références

Eur Urol. 2019 Nov;76(5):639-657
pubmed: 31443960
J Mol Diagn. 2016 Jan;18(1):15-22
pubmed: 26586227
J Surg Oncol. 2015 Jun;111(8):992-9
pubmed: 25975233
Nat Rev Mol Cell Biol. 2014 Mar;15(3):178-96
pubmed: 24556840
Int J Cancer. 2019 Apr 15;144(8):1941-1953
pubmed: 30350310
Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3713-8
pubmed: 21300873
Int J Mol Sci. 2018 Aug 24;19(9):
pubmed: 30149597
Int J Mol Sci. 2017 Oct 02;18(10):
pubmed: 29036883
Int J Mol Cell Med. 2015 Winter;4(1):32-9
pubmed: 25815280
Ann Surg. 2014 Apr;259(4):735-43
pubmed: 23982750
Cell Death Dis. 2017 Oct 12;8(10):e3100
pubmed: 29022903
J Cancer. 2016 Aug 7;7(12):1740-1746
pubmed: 27698912
Oncol Lett. 2019 Nov;18(5):5549-5554
pubmed: 31612063
Biomed Res Int. 2017;2017:1928021
pubmed: 28321402
Urol Oncol. 2010 Nov-Dec;28(6):655-61
pubmed: 19375957
J Exp Clin Cancer Res. 2016 Nov 7;35(1):173
pubmed: 27821145
Biomark Med. 2018 Jun;12(6):667-676
pubmed: 29896971
Int J Mol Sci. 2020 May 27;21(11):
pubmed: 32471285
J Exp Clin Cancer Res. 2014 Dec 31;33:779
pubmed: 25551284
Dis Markers. 2018 Jul 4;2018:6071826
pubmed: 30069274
Br J Cancer. 2016 Feb 16;114(4):454-62
pubmed: 26812572
Clin Genitourin Cancer. 2012 Jun;10(2):106-13
pubmed: 22386240
Exp Ther Med. 2017 Jul;14(1):10-17
pubmed: 28672887
Int J Cancer. 2009 May 1;124(9):2236-42
pubmed: 19127597
Mol Cancer. 2014 Jan 15;13:8
pubmed: 24423412
Int J Cancer. 2019 Jan 15;144(2):380-388
pubmed: 30183088
PLoS One. 2011;6(9):e24145
pubmed: 21909417
Br J Cancer. 2012 Jun 26;107(1):123-8
pubmed: 22644299
Cancer Res. 2009 Nov 1;69(21):8472-81
pubmed: 19843843
Methods Mol Biol. 2017;1660:111-130
pubmed: 28828652
Int J Cancer. 2018 Jun 15;142(12):2501-2511
pubmed: 29388209
Br J Cancer. 2016 Jun 14;114(12):1362-6
pubmed: 27228285
Cancer Sci. 2011 Mar;102(3):522-9
pubmed: 21166959
J Transl Med. 2020 Feb 3;18(1):51
pubmed: 32014049
Oncotarget. 2017 Jun 20;8(25):40832-40842
pubmed: 28388561
Oncotarget. 2015 Dec 8;6(39):42258-75
pubmed: 26517683
Front Oncol. 2018 Sep 07;8:362
pubmed: 30245975
Cancer Res. 2009 Jun 1;69(11):4851-60
pubmed: 19487295
Int J Cancer. 2017 Jan 1;140(1):176-187
pubmed: 27632639
Oncol Lett. 2018 Oct;16(4):4114-4128
pubmed: 30250529
Mol Biol Cell. 2011 May 15;22(10):1686-98
pubmed: 21411626
Cell Physiol Biochem. 2018;48(1):87-98
pubmed: 30001529
Nat Rev Cancer. 2015 Jan;15(1):25-41
pubmed: 25533674
Carcinogenesis. 2012 Apr;33(4):828-34
pubmed: 22298638

Auteurs

Ilaria Cavallari (I)

Veneto Institute of Oncology IOV-IRCCS, 35128, Padua, Italy.

Angela Grassi (A)

Veneto Institute of Oncology IOV-IRCCS, 35128, Padua, Italy.

Paola Del Bianco (P)

Veneto Institute of Oncology IOV-IRCCS, 35128, Padua, Italy.

Alberto Aceti (A)

Department of Surgery, Oncology and Gastroenterology, University of Padua, 35128 Padua, Italy.

Carlotta Zaborra (C)

Department of Surgery, Oncology and Gastroenterology, University of Padua, 35128 Padua, Italy.

Evgeniya Sharova (E)

Veneto Institute of Oncology IOV-IRCCS, 35128, Padua, Italy.

Irene Bertazzolo (I)

Veneto Institute of Oncology IOV-IRCCS, 35128, Padua, Italy.

Donna M D'Agostino (DM)

Department of Biomedical Sciences, University of Padua, 35131 Padua, Italy.

Massimo Iafrate (M)

Department of Surgery, Oncology and Gastroenterology, University of Padua, 35128 Padua, Italy.

Vincenzo Ciminale (V)

Veneto Institute of Oncology IOV-IRCCS, 35128, Padua, Italy.
Department of Surgery, Oncology and Gastroenterology, University of Padua, 35128 Padua, Italy.

Classifications MeSH