A Four-Dimensional Organoid System to Visualize Cancer Cell Vascular Invasion.
4D culture
exosome
mimic model
tight junction
vascular invasion
vascular organoid
Journal
Biology
ISSN: 2079-7737
Titre abrégé: Biology (Basel)
Pays: Switzerland
ID NLM: 101587988
Informations de publication
Date de publication:
27 Oct 2020
27 Oct 2020
Historique:
received:
05
09
2020
revised:
08
10
2020
accepted:
22
10
2020
entrez:
30
10
2020
pubmed:
31
10
2020
medline:
31
10
2020
Statut:
epublish
Résumé
Vascular invasion of cancer is a critical step in cancer progression, but no drug has been developed to inhibit vascular invasion. To achieve the eradication of cancer metastasis, elucidation of the mechanism for vascular invasion and the development of innovative treatment methods are required. Here, a simple and reproducible vascular invasion model is established using a vascular organoid culture in a fibrin gel with collagen microfibers. Using this model, it was possible to observe and evaluate the cell dynamics and histological positional relationship of invasive cancer cells in four dimensions. Cancer-derived exosomes promoted the vascular invasion of cancer cells and loosened tight junctions in the vascular endothelium. As a new evaluation method, research using this vascular invasion mimic model will be advanced, and applications to the evaluation of the vascular invasion suppression effect of a drug are expected.
Identifiants
pubmed: 33120912
pii: biology9110361
doi: 10.3390/biology9110361
pmc: PMC7692192
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 15H05791; 17H02099; 17H04282; 17K19698; 18K16356; 18K16355; 18KK0251; 19K22658; 19K09172; 19K07688
Organisme : Japan Agency for Medical Research and Development
ID : 16cm0106414h0001; 17cm0106414h0002
Références
Int J Biol Macromol. 1992 Jun;14(3):129-38
pubmed: 1390444
Curr Biol. 2018 Apr 23;28(8):R435-R444
pubmed: 29689228
Nat Rev Cancer. 2013 Dec;13(12):858-70
pubmed: 24263189
J Exp Clin Cancer Res. 2019 Mar 22;38(1):117
pubmed: 30898166
Biofabrication. 2017 Sep 01;9(4):045001
pubmed: 28812983
Int J Biochem Cell Biol. 2012 Jan;44(1):11-5
pubmed: 22024155
Mol Biol Cell. 2017 Jan 1;28(1):85-97
pubmed: 27852896
Cell Chem Biol. 2017 Sep 21;24(9):1092-1100
pubmed: 28757181
Biomaterials. 2018 Oct;179:144-155
pubmed: 29986232
Int J Mol Sci. 2017 Aug 17;18(8):
pubmed: 28817103
ACS Appl Mater Interfaces. 2016 Oct 5;8(39):25840-25847
pubmed: 27606718
Cancer Res. 2017 Dec 1;77(23):6480-6488
pubmed: 29162616
Nat Rev Cancer. 2016 Aug 23;16(9):582-98
pubmed: 27550820
J Proteomics. 2010 Sep 10;73(10):1907-20
pubmed: 20601276
Ann Gastroenterol Surg. 2017 Apr 25;1(1):5-10
pubmed: 29863129
Mol Biol Cell. 2017 Jan 1;28(1):30-40
pubmed: 27798235
Cell Mol Gastroenterol Hepatol. 2017 Jan 20;3(2):163-173
pubmed: 28275683
Pharmacol Res Perspect. 2017 Aug;5(4):
pubmed: 28805976
Nat Rev Cancer. 2019 Feb;19(2):65-81
pubmed: 30647431
Int J Mol Sci. 2018 Jan 18;19(1):
pubmed: 29346265
Nat Protoc. 2017 May;12(5):865-880
pubmed: 28358393
Hepatology. 2018 Oct;68(4):1459-1475
pubmed: 29637568
Cancer Lett. 2016 Oct 1;380(2):534-44
pubmed: 25449784
Cell Mol Life Sci. 2017 Feb;74(4):697-713
pubmed: 27582126
J Cell Physiol. 2017 Oct;232(10):2679-2697
pubmed: 27791270
Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):214-9
pubmed: 25524628
World J Gastroenterol. 2013 Aug 21;19(31):5051-60
pubmed: 23964138
Cell. 2007 Aug 24;130(4):601-10
pubmed: 17719539
Oncotarget. 2017 Jan 10;8(2):3683-3695
pubmed: 27713136
Int J Mol Sci. 2019 May 26;20(10):
pubmed: 31130715
Int J Mol Sci. 2019 Feb 15;20(4):
pubmed: 30781344
Gut. 2017 Apr;66(4):683-691
pubmed: 26818619
J Mol Med (Berl). 2013 Apr;91(4):431-7
pubmed: 23519402
Methods Mol Biol. 2018;1699:179-186
pubmed: 29086377
J Mater Sci Mater Med. 2015 May;26(5):180
pubmed: 25893395
Cancer Res. 1988 Dec 1;48(23):6863-71
pubmed: 2846163
Biochem Biophys Res Commun. 2005 Jul 29;333(2):328-35
pubmed: 15961063
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593