Size-Exclusion Chromatography as a Technique for the Investigation of Novel Extracellular Vesicles in Cancer.
SEC
biomarkers
cancer
exosomes
extracellular vesicles
size-exclusion chromatography
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
27 Oct 2020
27 Oct 2020
Historique:
received:
10
09
2020
revised:
06
10
2020
accepted:
15
10
2020
entrez:
30
10
2020
pubmed:
31
10
2020
medline:
31
10
2020
Statut:
epublish
Résumé
Cancer cells release extracellular vesicles, which are a rich target for biomarker discovery and provide a promising mechanism for liquid biopsy. Size-exclusion chromatography (SEC) is an increasingly popular technique, which has been rediscovered for the purposes of extracellular vesicle (EV) isolation and purification from diverse biofluids. A systematic review was undertaken to identify all papers that described size exclusion as their primary EV isolation method in cancer research. In all, 37 papers were identified and discussed, which showcases the breadth of applications in which EVs can be utilised, from proteomics, to RNA, and through to functionality. A range of different methods are highlighted, with Sepharose-based techniques predominating. EVs isolated using SEC are able to identify cancer cells, highlight active pathways in tumourigenesis, clinically distinguish cohorts, and remain functionally active for further experiments.
Identifiants
pubmed: 33121160
pii: cancers12113156
doi: 10.3390/cancers12113156
pmc: PMC7693800
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Subventions
Organisme : Royal College of Surgeons in England
ID : -
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