External female genitalia of Triatoma jatai, Triatoma costalimai and Triatoma williami (Hemiptera: Reduviidae: Triatominae).
Genitalia
Scanning electron microscopy
Taxonomy
Triatomines
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
29 Oct 2020
29 Oct 2020
Historique:
received:
03
08
2020
accepted:
21
10
2020
entrez:
30
10
2020
pubmed:
31
10
2020
medline:
14
7
2021
Statut:
epublish
Résumé
Taxonomic identification of triatomines is generally performed based on aspects of their external morphology. However, the use of a multidisciplinary approach, considering morphological aspects of the external genitalia, morphometry, genetics, and phylogeography has been suggested, especially for similar and/or cryptic species. The rupestral species Triatoma jatai Gonçalves et al., 2013, Triatoma costalimai Verano & Galvão, 1959 and Triatoma williami Galvão et al., 1965, which are morphologically similar, have been found naturally infected with Trypanosoma cruzi (Chagas, 1909) in wild, peridomestic, and intradomestic environments, representing a risk of new outbreaks of Chagas disease. This study presents morphological description complementation of these species, with an emphasis on the structures of the female external genitalia, using scanning electron microscopy. The females of T. jatai and T. costalimai (n = 10 of each) were captured in the Brazilian municipalities of Paranã and Aurora do Tocantins and were identified with the use of a dichotomous key for the Matogrossensis subcomplex. Females of T. williami (n = 5), were obtained from a laboratory colony. The females were cut transversely at the sixth abdominal segment and examined under scanning electron microscopy (SEM) at the Oswaldo Cruz/Fiocruz Institute Electronic Microscopy Platform. It was possible to differentiate the three species based on the characteristics of urotergites VII, VIII and IX and urosternite VII, as well as the genital plaques, gonocoxites, and gonapophyses. To our knowledge, morphological differences in the spines present on gonapophysis 8 in triatomines are described here for the first time. The results show that external genitalia of females are useful structures to differentiate T. costalimai, T. jatai and T. williami. SEM analysis contributes to and corroborates, together with other tools morphological and molecular, the distinction of the three species.
Sections du résumé
BACKGROUND
BACKGROUND
Taxonomic identification of triatomines is generally performed based on aspects of their external morphology. However, the use of a multidisciplinary approach, considering morphological aspects of the external genitalia, morphometry, genetics, and phylogeography has been suggested, especially for similar and/or cryptic species. The rupestral species Triatoma jatai Gonçalves et al., 2013, Triatoma costalimai Verano & Galvão, 1959 and Triatoma williami Galvão et al., 1965, which are morphologically similar, have been found naturally infected with Trypanosoma cruzi (Chagas, 1909) in wild, peridomestic, and intradomestic environments, representing a risk of new outbreaks of Chagas disease. This study presents morphological description complementation of these species, with an emphasis on the structures of the female external genitalia, using scanning electron microscopy.
METHODS
METHODS
The females of T. jatai and T. costalimai (n = 10 of each) were captured in the Brazilian municipalities of Paranã and Aurora do Tocantins and were identified with the use of a dichotomous key for the Matogrossensis subcomplex. Females of T. williami (n = 5), were obtained from a laboratory colony. The females were cut transversely at the sixth abdominal segment and examined under scanning electron microscopy (SEM) at the Oswaldo Cruz/Fiocruz Institute Electronic Microscopy Platform.
RESULTS
RESULTS
It was possible to differentiate the three species based on the characteristics of urotergites VII, VIII and IX and urosternite VII, as well as the genital plaques, gonocoxites, and gonapophyses. To our knowledge, morphological differences in the spines present on gonapophysis 8 in triatomines are described here for the first time.
CONCLUSIONS
CONCLUSIONS
The results show that external genitalia of females are useful structures to differentiate T. costalimai, T. jatai and T. williami. SEM analysis contributes to and corroborates, together with other tools morphological and molecular, the distinction of the three species.
Identifiants
pubmed: 33121526
doi: 10.1186/s13071-020-04418-2
pii: 10.1186/s13071-020-04418-2
pmc: PMC7597029
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
538Références
J Med Entomol. 2017 Nov 7;54(6):1771-1774
pubmed: 29106640
J Vector Ecol. 2017 Dec;42(2):271-278
pubmed: 29125242
Am J Trop Med Hyg. 2013 Oct;89(4):775-80
pubmed: 23958911
Zookeys. 2016 Oct 3;(621):45-62
pubmed: 27833419
Parasit Vectors. 2014 Jan 09;7:17
pubmed: 24405517
Trans R Soc Trop Med Hyg. 1999 Jan-Feb;93(1):13-4
pubmed: 10492778
Acta Trop. 2009 May-Jun;110(2-3):88-100
pubmed: 19385053
Mem Inst Oswaldo Cruz. 2010 May;105(3):286-92
pubmed: 20512241
J Vector Ecol. 2020 Jun;45(1):57-68
pubmed: 32492272
Acta Trop. 2018 Sep;185:86-97
pubmed: 29684355
Am J Trop Med Hyg. 2019 Jan;100(1):90-92
pubmed: 30426923
Adv Parasitol. 2018;99:265-344
pubmed: 29530308
PLoS Negl Trop Dis. 2017 Nov 16;11(11):e0006035
pubmed: 29145405
Parasit Vectors. 2014 Mar 31;7:149
pubmed: 24685273
Am J Trop Med Hyg. 2016 Mar;94(3):686-8
pubmed: 26787157
Zookeys. 2017 May 18;(675):1-25
pubmed: 28769676
Mem Inst Oswaldo Cruz. 2013 Jun;108(4):429-37
pubmed: 23828010
Infect Genet Evol. 2016 Sep;43:225-31
pubmed: 27245153