Cytological analysis of bronchoalveolar lavage fluid in asbestos-exposed workers.


Journal

La Medicina del lavoro
ISSN: 0025-7818
Titre abrégé: Med Lav
Pays: Italy
ID NLM: 0401176

Informations de publication

Date de publication:
31 Oct 2020
Historique:
received: 10 04 2020
accepted: 24 07 2020
entrez: 30 10 2020
pubmed: 31 10 2020
medline: 21 11 2020
Statut: epublish

Résumé

Asbestos-related lung diseases are a group of heterogeneous disorders with different pathogenesis and prognosis. Very few studies investigated the BALF cell profile of asbestos exposed workers. The existence of a relationship between bronchoalveolar lavage fluid (BALF) cellular pattern and specific diagnosis and/or asbestos exposure biomarkers would allow the identification of effect biomarkers useful in the follow up of asbestos-exposed workers and in the diagnosis of asbestos-related diseases. To assess BALF cell profile in formerly asbestos-exposed workers and its relationship with asbestos fibre (amphibole and chrysotile) and asbestos body (AB) concentrations. 113 male workers formerly exposed to asbestos underwent bronchoscopy with bronchoalveolar lavage and were retrospectively enrolled. 35 of them were affected by pleural plaques and 10 were affected by asbestosis. Pulmonary functional tests (PFT), BALF cellular pattern, BALF mineralogical analysis with asbestos fibres and AB counting were performed in each patient. A statistical analysis with a multivariate linear regression model was adopted. From the statistical analysis of data a direct correlation between pack-years and BALF macrophages was found. Inversely correlation between pack-years and BALF lymphocytes was detected. There was not relationship among BALF cellular pattern, PFT values, specific diagnosis, BALF AB count or BALF asbestos fibre concentration. BALF cellular pattern does not seem to be related to asbestos exposure biomarkers like AB and asbestos fibre concentration in BALF. Instead, smoke habit can induce an increase in BALF macrophages and a decrease of BALF lymphocytes count.

Sections du résumé

BACKGROUND BACKGROUND
Asbestos-related lung diseases are a group of heterogeneous disorders with different pathogenesis and prognosis. Very few studies investigated the BALF cell profile of asbestos exposed workers. The existence of a relationship between bronchoalveolar lavage fluid (BALF) cellular pattern and specific diagnosis and/or asbestos exposure biomarkers would allow the identification of effect biomarkers useful in the follow up of asbestos-exposed workers and in the diagnosis of asbestos-related diseases.
OBJECTIVES OBJECTIVE
To assess BALF cell profile in formerly asbestos-exposed workers and its relationship with asbestos fibre (amphibole and chrysotile) and asbestos body (AB) concentrations.
METHODS METHODS
113 male workers formerly exposed to asbestos underwent bronchoscopy with bronchoalveolar lavage and were retrospectively enrolled. 35 of them were affected by pleural plaques and 10 were affected by asbestosis. Pulmonary functional tests (PFT), BALF cellular pattern, BALF mineralogical analysis with asbestos fibres and AB counting were performed in each patient. A statistical analysis with a multivariate linear regression model was adopted.
RESULTS RESULTS
From the statistical analysis of data a direct correlation between pack-years and BALF macrophages was found. Inversely correlation between pack-years and BALF lymphocytes was detected. There was not relationship among BALF cellular pattern, PFT values, specific diagnosis, BALF AB count or BALF asbestos fibre concentration.
DISCUSSION CONCLUSIONS
BALF cellular pattern does not seem to be related to asbestos exposure biomarkers like AB and asbestos fibre concentration in BALF. Instead, smoke habit can induce an increase in BALF macrophages and a decrease of BALF lymphocytes count.

Identifiants

pubmed: 33124609
doi: 10.23749/mdl.v111i5.9170
pmc: PMC7809980
doi:

Substances chimiques

Asbestos 1332-21-4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

379-387

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Auteurs

Pietro Sartorelli (P)

Department of Medical Biotechnology, University of Siena, Unit of Occupational Health, Siena University Hospital, Viale Bracci 16, 53100 Siena, Italy. pietro.sartorelli@unisi.it.

Sveva Indini (S)

Department of Medical Biotechnology, University of Siena, Unit of Occupational Health, Siena University Hospital, Viale Bracci 16, 53100 Siena, Italy. sveva.indini@gmail.com.

Francesco Bianchi (F)

Department of Medical, Surgical and Neurological Sciences, University of Siena, Unit of Respiratory Disease and Lung Transplant, Siena University Hospital, Viale Bracci 16, 53100 Siena, Italy. bianchicecco.1990@gmail.com.

Miriana D'Alessandro (M)

Department of Medical, Surgical and Neurological Sciences, University of Siena, Unit of Respiratory Disease and Lung Transplant, Siena University Hospital, Viale Bracci 16, 53100 Siena, Italy. dalessandro.miriana@gmail.com.

Laura Bergantini (L)

Department of Medical, Surgical and Neurological Sciences, University of Siena, Unit of Respiratory Disease and Lung Transplant, Siena University Hospital, Viale Bracci 16, 53100 Siena, Italy. laurabergantini@gmail.com.

Paolo Cameli (P)

Department of Medical, Surgical and Neurological Sciences, University of Siena, Unit of Respiratory Disease and Lung Transplant, Siena University Hospital, Viale Bracci 16, 53100 Siena, Italy. paolo.cameli@yahoo.com.

Maria Antonietta Mazzei (MA)

Department of Medical Surgical and Neurological Sciences, University of Siena, Unit of Diagnostic Imaging, Siena University Hospital, Viale Bracci 16, 53100 Siena, Italy. mariaantonietta.mazzei@unisi.it.

Giuseppina Scancarello (G)

Unit of Occupational Hygiene and Toxicology, Laboratory of Public Health AUSL South-East Tuscany, Strada del Ruffolo 4, 53100 Siena, Italy. giuseppina.scancarello@uslsudest.toscana.it.

Lucio Barabesi (L)

Department of Economics and Statistics, University of Siena, Piazza San Francesco 7, 53100 Siena, Italy. lucio.barabesi@unisi.it.

Elena Bargagli (E)

Department of Medical, Surgical and Neurological Sciences, University of Siena, Unit of Respiratory Disease and Lung Transplant, Siena University Hospital, Viale Bracci 16, 53100 Siena, Italy. elena.bargagli@unisi.it.

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