The New and the Old: Platform Cross-Validation of Immunoaffinity MASS Spectrometry versus ELISA for PromarkerD, a Predictive Test for Diabetic Kidney Disease.
ELISA
MRM
antibodies
assay development
biomarkers
diabetic kidney disease
immunoaffinity
multiplex
targeted mass spectrometry
Journal
Proteomes
ISSN: 2227-7382
Titre abrégé: Proteomes
Pays: Switzerland
ID NLM: 101621966
Informations de publication
Date de publication:
28 Oct 2020
28 Oct 2020
Historique:
received:
30
09
2020
revised:
22
10
2020
accepted:
27
10
2020
entrez:
31
10
2020
pubmed:
1
11
2020
medline:
1
11
2020
Statut:
epublish
Résumé
PromarkerD is a proteomics derived test for predicting diabetic kidney disease that measures the concentrations of three plasma protein biomarkers, APOA4, CD5L and IBP3. Antibodies against these proteins were developed and applied to a multiplexed immunoaffinity capture mass spectrometry assay. In parallel, and facilitating current clinical laboratory workflows, a standard ELISA was also developed to measure each protein. The performance characteristics of the two technology platforms were compared using a cohort of 100 samples, with PromarkerD test scores demonstrating a high correlation (R = 0.97). These technologies illustrate the potential for large scale, high throughput clinical applications of proteomics now and into the future.
Identifiants
pubmed: 33126588
pii: proteomes8040031
doi: 10.3390/proteomes8040031
pmc: PMC7709118
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
J Diabetes Complications. 2019 Dec;33(12):107406
pubmed: 31669066
Expert Rev Proteomics. 2020 Apr;17(4):257-273
pubmed: 32427033
Proteomics. 2019 May;19(10):e1700448
pubmed: 30353665
Clin Transl Med. 2014 Mar 29;3(1):7
pubmed: 24679154
Crit Rev Clin Lab Sci. 2020 Jun 19;:1-12
pubmed: 32552254
Clin Proteomics. 2020 Oct 20;17:37
pubmed: 33093819
Clin J Am Soc Nephrol. 2015 Aug 7;10(8):1371-9
pubmed: 26175542
Immunochemistry. 1971 Sep;8(9):871-4
pubmed: 5135623
Am J Obstet Gynecol. 2016 Jul;215(1):82.e1-82.e11
pubmed: 26970494
Acta Pharmacol Sin. 2018 Jul;39(7):1068-1072
pubmed: 29645001
Nat Rev Cancer. 2017 Mar;17(3):199-204
pubmed: 28154374
Clin Proteomics. 2013 Oct 02;10(1):13
pubmed: 24088261
Int J Epidemiol. 2013 Apr;42(2):412-21
pubmed: 22544845
FEBS Lett. 1971 Jun 24;15(3):232-236
pubmed: 11945853
Diabetes Care. 2017 Nov;40(11):1548-1555
pubmed: 28851702
Lancet. 1986 Feb 8;1(8476):307-10
pubmed: 2868172
Oncologist. 2019 Jun;24(6):e251-e259
pubmed: 30139835
EuPA Open Proteom. 2017 Jan 05;14:1-10
pubmed: 29900119