Development of a SARS-CoV-2 Total Antibody Assay and the Dynamics of Antibody Response over Time in Hospitalized and Nonhospitalized Patients with COVID-19.

Adult Antibodies, Viral / immunology Antibody Formation COVID-19 / diagnosis COVID-19 Nucleic Acid Testing COVID-19 Serological Testing Convalescence Female Humans Immunologic Tests Male Middle Aged Nucleocapsid Proteins / immunology SARS-CoV-2 / immunology

Journal

Journal of immunology (Baltimore, Md. : 1950)

ISSN: 1550-6606

Titre abrégé: J Immunol

Pays: United States

ID NLM: 2985117R

Informations de publication

Date de publication:
15 12 2020

Historique:

received: 06 07 2020

accepted: 08 10 2020

pubmed: 1 11 2020

medline: 22 12 2020

entrez: 31 10 2020

Statut: ppublish

Résumé

Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infections often cause only mild disease that may evoke relatively low Ab titers compared with patients admitted to hospitals. Generally, total Ab bridging assays combine good sensitivity with high specificity. Therefore, we developed sensitive total Ab bridging assays for detection of SARS-CoV-2 Abs to the receptor-binding domain (RBD) and nucleocapsid protein in addition to conventional isotype-specific assays. Ab kinetics was assessed in PCR-confirmed, hospitalized coronavirus disease 2019 (COVID-19) patients (

Identifiants

DOI: 10.4049/jimmunol.2000767 PMID: 33127820

pubmed: 33127820

pii: jimmunol.2000767

doi: 10.4049/jimmunol.2000767

doi:

Substances chimiques

Antibodies, Viral 0

Nucleocapsid Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

3491-3499

Investigateurs

Robert Hemke (R)

Diederik van de Beek (D)

Martijn Beudel (M)

Matthijs C Brouwer (MC)

Bart Geerts (B)

Markus Hollmann (M)

Bennedikt Preckel (B)

Denise Veelo (D)

A H Koos Zwinderman (AHK)

Theo Geijtenbeek (T)

Florianne Hafkamp (F)

Diane Bax (D)

Alex Cloherty (A)

Michiel van Agtmael (M)

Marije Bomers (M)

Suzanne Geerlings (S)

Martin P Grobusch (MP)

Vanessa Harris (V)

Sabine M Hermans (SM)

Joppe W Hovius (JW)

Jeaninne Nellen (J)

Edgar Peters (E)

Tom van der Poll (T)

Jan M Prins (JM)

Kim Sigaloff (K)

Cornelis S Stijnis (CS)

Marc van der Valk (M)

Michèle van Vugt (M)

W Joost Wiersinga (WJ)

Godelieve Bree (G)

Alexander P J Vlaar (APJ)

Sanne de Bruin (S)

Anne Geke Algera (AG)

Frank van Baarle (F)

Lieuwe Bos (L)

Michela Botta (M)

Esther Bulle (E)

Paul Elbers (P)

Lucas Fleuren (L)

Armand Girbes (A)

Laura Hagens (L)

Leo Heunks (L)

Janneke Horn (J)

Mourik van Mourik (M)

Frederique Paulus (F)

Jorinde Raasveld (J)

Marcus Schultz (M)

Marry Smit (M)

Willemke Stilma (W)

Patrick Thoral (P)

Anissa Tsonas (A)

Heder de Vries (H)

Alex Schuurmans (A)

Menno de Jong (M)

Marianna Bugiani (M)

Harm Jan Bogaard (HJ)

Charlotte Teunissen (C)

Jorg Hamann (J)