HIV influences clustering and intracellular replication of hepatitis C virus.
biopsy
coinfection
mathematical model
single-cell laser-capture microdissection
spatial
Journal
Journal of viral hepatitis
ISSN: 1365-2893
Titre abrégé: J Viral Hepat
Pays: England
ID NLM: 9435672
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
25
06
2020
revised:
21
08
2020
accepted:
12
10
2020
pubmed:
1
11
2020
medline:
1
9
2021
entrez:
31
10
2020
Statut:
ppublish
Résumé
HCV and HIV coinfection is common and HIV leads to increased HCV viraemia and accelerated disease progression. However, the biological basis of this interaction remains poorly understood and little is known about the impact of HIV on HCV replication at the cellular level. We analysed HCV RNA, based on single-cell laser-capture microdissection, in liver biopsies from monoinfected (n = 4) and HCV/HIV-coinfected (n = 5) participants. HCV RNA was assayed in 3200 hepatocytes with information of spatial position. We compared HCV RNA levels and clustering properties of infection between mono- and coinfected participants, and developed a mathematical model of infection. Although the median plasma HCV RNA level and the fraction of infected cells were comparable in monoinfected (7.0 log
Identifiants
pubmed: 33128322
doi: 10.1111/jvh.13429
pmc: PMC7855861
mid: NIHMS1653188
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
334-344Subventions
Organisme : NIH HHS
ID : R01 OD011095
Pays : United States
Organisme : NIDA NIH HHS
ID : DA016078
Pays : United States
Organisme : National Institute of Allergy and Infectious Diseases
ID : AI116868
Organisme : NIH Office of the Director
ID : OD011095
Organisme : NIAID NIH HHS
ID : R01 AI078881
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116868
Pays : United States
Organisme : National Institute of Allergy and Infectious Diseases
ID : AI078881
Organisme : NIDA NIH HHS
ID : R01 DA013806
Pays : United States
Organisme : National Institute of Allergy and Infectious Diseases
ID : AI028433
Organisme : NIDA NIH HHS
ID : R01 DA016078
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI028433
Pays : United States
Informations de copyright
Published 2020. This article is a U.S. Government work and is in the public domain in the USA.
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