Z

Calcium / chemistry Chromatography, Affinity Humans Hydrogen-Ion Concentration Immunoglobulin G / chemistry Protein Domains Staphylococcal Protein A / chemistry
Antibody purification Calcium Elution Ligand Protein A ZCa pH

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2021
Historique:
entrez: 31 10 2020
pubmed: 1 11 2020
medline: 24 3 2021
Statut: ppublish

Résumé

Therapeutic antibodies are at the forefront of modern medicine where high purity, which is typically obtained by Protein A-based affinity purification, is of utmost importance. In this chapter, we present a method for neutral and selective purification of antibodies by utilizing an engineered affinity ligand, Z

Identifiants

DOI: 10.1007/978-1-0716-0775-6_17 PMID: 33128754
pubmed: 33128754
doi: 10.1007/978-1-0716-0775-6_17
doi:

Substances chimiques

Immunoglobulin G 0
Staphylococcal Protein A 0
Calcium SY7Q814VUP

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

245-249

Auteurs

Julia Scheffel (J)

Department of Protein Science, KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), AlbaNova University Centre, Stockholm, Sweden.

Sara Kanje (S)

Department of Protein Science, KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), AlbaNova University Centre, Stockholm, Sweden.

Sophia Hober (S)

Department of Protein Science, KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), AlbaNova University Centre, Stockholm, Sweden. sophia@biotech.kth.se.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Calcium › Z
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'affinité › Z
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Z
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Z
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Isotypes des immunoglobulines › Immunoglobuline G › Z
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Z
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes bactériens › Protéine A staphylococcique › Z